Noninvasive Delayed Limb Ischemic Preconditioning in Rats Increases Antioxidant Activities in Cerebral Tissue during Severe Ischemia-Reperfusion Injury
Noninvasive Delayed Limb Ischemic Preconditioning in Rats Increases Antioxidant Activities in Cerebral Tissue during Severe Ischemia-Reperfusion Injury
复制标题
大鼠无创延迟肢体缺血预处理可增加严重缺血再灌注损伤期间脑组织的抗氧化活性
DOI：
10.1016/j.jss.2010.11.001
复制
发表时间：
2012-05-01
影响因子：
2.2
通讯作者：
Lou, Jian-Shi
中科院分区：
文献类型：
--
作者：
Yuan, Heng-Jie;Zhu, Xue-Hui;Lou, Jian-Shi
背景目的：研究无创性延迟肢体缺血预处理（NDLIP）对大鼠脑缺血再灌注（I/R）损伤的保护作用。健康雄性Wistar大鼠随机分为4组。以神经行为学指标和脑组织抗氧化能力的变化来评价NDLIP的延迟保护作用。通过观察神经行为研究神经功能。分光光度计测定脑组织总超氧化物歧化酶（T-SOD）、锰超氧化物歧化酶（Mn-SOD）、谷胱甘肽过氧化物酶（GSH-PX）、黄嘌呤氧化酶（XOD）活性及丙二醛（MDA）含量。采用逆转录聚合酶链反应（RT-PCR）方法检测Mn-SOD mRNA的表达。与I/R组相比，早期脑缺血预处理（ECIP）+ DI/R组和NDLIP + I/R组的脑梗死范围明显缩小（P < 0.05）。ECIP + I/R组和NDLIP + I/R组皮质和海马抗氧化酶活性和Mn-SOD表达均增加。ECIP + I/R组和NDLIP + I/R组大鼠大脑皮层和海马XOD活性和MDA含量明显降低。NDLIP可减少脑梗死面积，提高脑I/R损伤后的抗氧化能力，减轻过氧化损伤。NDLIP提供的抗氧化保护作用与ECIP一样有效。(C)2012 Elsevier Inc. All rights reserved.
Background. To study the protection offered by noninvasive delayed limb ischemic preconditioning (NDLIP) against cerebral ischemia reperfusion (I/R) injury in rats.Materials and Methods. Healthy male Wistar rats were randomly divided into four groups. The delayed protection offered by NDLIP was estimated in light of changes in the neural behavior marker and cerebral tissue antioxidative ability. Neurological functions were studied by observing neural behavior. Total superoxide dismutase (T-SOD), manganese-superoxide dismutase (Mn-SOD), glutathione peroxidase (GSH-PX), and xanthine oxidase (XOD) activity in cerebral tissue and malonaldehyde (MDA) content were detected using a spectrophotometer. Mn-SOD mRNA was measured by the reverse transcription polymerase chain reaction method.Results. Cerebral infarct size was diminished in the early cerebral ischemia preconditioning (ECIP) + DI/R and NDLIP + I/R groups compared with the I/R group (P < 0.05). The cortical and hippocampal antioxidant enzyme activity and Mn-SOD expression were increased in the ECIP + I/R and NDLIP + I/R groups. In contrast, the cortical and hippocampal XOD activity and MDA content decreased in the ECIP + I/R and NDLIP + I/R groups.Conclusions. NDLIP decreased cerebral infarct size, increased cerebral antioxidative ability after I/R injury, and decreased peroxidative damage. The antioxidative protection offered by NDLIP was as effective as that offered by ECIP. (C) 2012 Elsevier Inc. All rights reserved.