Alteration in the expression of inflammatory cytokines in primary hippocampal astrocytes in response to MK-801 through ERK1/2 and PI3K signals
Alteration in the expression of inflammatory cytokines in primary hippocampal astrocytes in response to MK-801 through ERK1/2 and PI3K signals
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DOI：
10.1016/j.cyto.2020.155366
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Wenjuan Yu
中科院分区：
文献类型：
--
作者：
Hao Zhu;Yu Yang;Min Zhu;Xiao Shi;Le Ye;Song Zhang;Hongwei Fang;Wenjuan Yu
Our previous study showed that dizocilpine (MK-801) induced schizophrenia-like behavior in rats, enhanced.GFAP expression, and activated primary cultured hippocampal astrocytes. Astrocytes play an essential role in.neuroinflammation and contribute to the crosstalk that generates chronic neuro-inflammation in neurological.diseases. However, the effects of MK-801 treatment on astrocytic neuroinflammatory responses and its mechanism of action have not been studied in detail. To address this issue, IL1β, IL6, TNFα and IL10 expression and.secretion levels were evaluated in hippocampal astrocytes in response to MK-801 for 24 h by ELISA and real-time.PCR, with and without pretreatment of either the ERK1/2 inhibitor, PD98059 or the PI3K inhibitor, LY294002..Cell apoptosis, viability, and proliferation were also examined. MK-801 treatment did not induce hippocampal.astrocytes apoptosis or proliferation, however, MK-801 enhanced astrocytes viability. Additionally, the expression and secretion levels of IL1β, IL6 and TNFα were elevated, but that of IL10 was decreased, in which ERK1/2.and PI3K signals were involved. These findings suggest that hippocampal astrocytes may regulate the expressions.of inflammatory cytokines through ERK1/2 and PI3K signaling pathway to participate in the pathogenesis of.schizophrenia.
