GAMMA-IRRADIATED SCRUB TYPHUS IMMUNOGENS - DEVELOPMENT AND DURATION OF IMMUNITY
GAMMA-IRRADIATED SCRUB TYPHUS IMMUNOGENS - DEVELOPMENT AND DURATION OF IMMUNITY
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DOI：
10.1128/iai.22.1.80-86.1978
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发表时间：
1978-01-01
影响因子：
3.1
通讯作者：
OSTERMAN, JV
中科院分区：
文献类型：
--
作者：
EISENBERG, GHG;OSTERMAN, JV
研究了接种. γ的BALB/c小鼠对致死性恙虫病感染的免疫发展和持续时间。-辐照的恙虫病立克次体，Karp菌株。一次含有约10850%小鼠致死剂量（MLD50）的辐照生物体的腹腔注射可引起免疫应答，抵抗105mld50活Karp的攻击。相同质量的免疫原每隔5天注射3次，使同源（Karp株）和异源（Kato株）的保护作用分别提高25倍和60倍。进一步扩大免疫方案的时间并没有增加保护作用。免疫s.c.提供了显著的保护水平，但低于i.p.免疫，但两种途径引起的细胞转移免疫水平大致相同。免疫接种后的免疫特异性保护迅速发展，足以抵抗200 MLD50卡普的同时攻击。同源免疫在3次注射方案完成后7天对106 MLD50攻击具有保护作用，在3个月后保持该水平，在9个月时降至104 MLD50，并在12个月时对50 MLD50 Karp攻击有效。第17天首次观察到对异源攻击的保护作用，第38天达到峰值，小鼠抵抗105 MLD50 Kato攻击。此后，异源保护迅速减弱，在6个月时不显著。
The development and duration of immunity to lethal scrub typhus infection was studied in BALB/c mice vaccinated with .gamma.-irradiated Rickettsia tsutsugamushi, strain Karp. One i.p. injection containing approximately 108 50% mouse lethal doses (MLD50) of irradiated organisms elicited an immune response protective against challenge with 105 MLD50 of viable Karp. The same mass of immunogen given in 3 injections at 5-day intervals increased homologous (Karp strain) protection 25-fold and heterologous (Kato strain) protection 60-fold. Further temporal expansion of the immunization regimen did not increase protection. Vaccination s.c. provided significant, but lower, levels of protection than were achieved by i.p. immunization, but the levels of cell-transferable immunity elicited by the 2 routes were approximately the same. Immunologically specific protection after i.p. vaccination developed rapidly enough to provide resistance against simultaneous challenge with 200 MLD50 of Karp. Homologous immunity was protective against a 106 MLD50 challenge 7 days after completion of the 3-injection regimen, remained at that level for 3 mo., dropped to 104 MLD50 by 9 mo. and was effective against a 50 MLD50 Karp challenge at 12 mo. Protection against heterologous challenge was first observed on day 17 and peaked on day 38, when the mice resisted a 105 MLD50 Kato challenge. Thereafter, heterologous protection waned rapidly and was not significant at 6 mo.