Assembly of spermatid acrosome depends on microtubule organization during mammalian spermiogenesis
Assembly of spermatid acrosome depends on microtubule organization during mammalian spermiogenesis
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DOI：
10.1016/j.ydbio.2006.02.001
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发表时间：
2006-05-01
影响因子：
2.7
通讯作者：
Schatten, G
中科院分区：
文献类型：
--
作者：
Moreno, RD;Palomino, J;Schatten, G
顶体是附着在精子核上的分泌囊泡。我们的假设是，微管参与的高尔基体和顶体之间的膜交通在精子细胞分化的第一步。在这项工作中，我们表明，诺考达唑诱导的微管去极化触发顶体膜囊泡的形成，而不脱离核膜的顶体。诺考达唑还诱导高尔基体的碎片，如通过抗巨噬细胞抗体、高尔基体蛋白-97和GM 130以及电子显微镜所确定的。相反，无论是顶体，也不是高尔基体经历了在延长精子细胞（顶体和成熟阶段）的碎片。azh/azh小鼠圆形精子细胞的微管网络也变得紊乱。解体相关的顶体和高尔基体的碎片，由域特异性标记进行评估。azh/azh小鼠的伸长精子细胞（顶体和成熟期）也有微管组织，顶体和高尔基体的改变。最后，azh/azh小鼠的精子显示异常定位的顶体蛋白sp 56在后顶体和鞭毛域。我们的研究结果表明，微管参与的fort-nation和/或维护的顶体和高尔基体的结构和微管的组织在圆形精子细胞是关键的顶体蛋白分选到适当的细胞器。(c)2006年爱思唯尔公司All rights reserved.
The acrosome is a secretory vesicle attached to the nucleus of the sperm. Our hypothesis is that microtubules participate in the membrane traffic between the Golgi apparatus and acrosome during the first steps of spermatid differentiation. In this work, we show that nocodazole-induced microtubule depolarization triggers the formation of vesicles of the acrosomal membrane, without detaching the acrosome from the nuclear envelope. Nocodazole also induced fragmentation of the Golgi apparatus as determined by antibodies against giantin, golgin-97 and GM130, and electron microscopy. Conversely, neither the acrosome nor the Golgi apparatus underwent fragmentation in elongating spermatids (acrosome- and maturation-phase). The microtubule network of round spermatids of azh/azh mice also became disorganized. Disorganization correlated with fragmentation of the acrosome and the Golgi apparatus, as evaluated by domain-specific markers. Elongating spermatids (acrosome and maturation-phase) of azh/azh mice also had alterations in microtubule organization, acrosome, and Golgi apparatus. Finally, the spermatozoa of azh/azh mice displayed aberrant localization of the acrosomal protein sp56 in both the post-acrosomal and flagellum domains. Our results suggest that microtubules participate in the fort-nation and/or maintenance of the structure of the acrosome and the Golgi apparatus and that the organization of the microtubules in round spermatids is key to sorting acrosomal proteins to the proper organelle. (c) 2006 Elsevier Inc. All rights reserved.