The application of atelocollagen gel in combination with porous scaffolds for cartilage tissue engineering and its suitable conditions
The application of atelocollagen gel in combination with porous scaffolds for cartilage tissue engineering and its suitable conditions
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DOI：
10.1002/jbm.a.32509
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发表时间：
2010-04-01
影响因子：
4.9
通讯作者：
Hoshi, K.
中科院分区：
文献类型：
--
作者：
Yamaoka, H.;Tanaka, Y.;Hoshi, K.
为了提高组织工程软骨的质量，我们检测了多孔体作为支架与人工胶原水凝胶结合的体内有效性，并探讨了工程组织中人工胶原和种子细胞的适宜条件。我们使用胶原海绵(CS)或含有人软骨细胞的多孔聚L-丙交酯(PLLA)和1%的水凝胶来构建组织工程化结构，其浓度可以最大限度地增加软骨基质的积累。CS较软，杨氏模数小于1 Mpa，而多孔PLLA很硬，杨氏模数为10 Mpa。尽管在裸鼠皮下移植2个月后，含有CS的构建物大小缩小到50%，但PLLA构建物保持其原始大小。在软骨细胞和水凝胶存在的情况下，两种多孔支架都有一定的软骨再生，但PLLA支架在体内显著积累了丰富的基质。在软骨细胞条件方面，软骨再生能力与第3~8代传代次数成反比，与细胞密度(10(6)~10(8)个/mL)呈线性关系。因此，硬质多孔支架在复合1%、胶原和部分条件软骨细胞时，可以保持组织工程软骨的大小，并实现体内较好的软骨再生。(C)2009年威利期刊公司J Bimed Mater res 93A：123-132,2010
For improving the quality of tissue-engineered cartilage, we examined the in vivo usefulness of porous bodies as scaffolds combined with an ateolocollagen hydrogel and investigated the suitable conditions for atelocollagen and seeding cells within the engineered tissues. We made tissue-engineered constructs using a collagen sponge (CS) or porous poly(L-lactide) (PLLA) with human chondrocytes and 1% hydrogel, the concentration of which maximized the accumulation of cartilage matrices. The CS was soft with a Young's modulus of less than 1 MPa, whereas the porous PLLA was very rigid with a Young's modulus of 10 MPa. Although the constructs with the CS shrank to 50% in size after a 2-month subcutaneous transplantation in nude mice, the PLLA constructs maintained their original sizes. Both of the porous scaffolds contained some cartilage regeneration in the presence of the chondrocytes and hydrogel, but the PLLA counterpart significantly accumulated abundant matrices in vivo. Regarding the conditions of the chondrocytes, the cartilage regeneration was improved in inverse proportion to the passage numbers among passages 3-8,and was linear with the cells densities (10(6) to 10(8) cells/mL). Thus, the rigid porous scaffold can maintain the size of the tissue-engineered cartilage and realize fair cartilage regeneration in vivo when combined with 1%, atelocollagen and some conditioned chondrocytes. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 123-132,2010