Selective transcription of vaccinia virus genes in template dependent soluble extracts of infected cells
Selective transcription of vaccinia virus genes in template dependent soluble extracts of infected cells
复制标题
受感染细胞模板依赖性可溶性提取物中痘苗病毒基因的选择性转录
DOI：
10.1016/0092-8674(83)90177-0
复制
发表时间：
1983
期刊：
影响因子：
64.5
通讯作者：
B. Moss
中科院分区：
文献类型：
--
作者：
C. Puckett;B. Moss
从感染细胞的裂解物中获得了特异性和准确地启动在确定的牛痘病毒模板上转录的可溶性系统。所需的调控信号包含在延伸至RNA起始位点上游约230 bp和下游约30 bp的DNA片段内。转录对α-鹅膏蕈碱有抗性，并被病毒RNA聚合酶的抗体抑制。未感染细胞的全细胞提取物不能准确地转录牛痘DNA。相反，在牛痘感染后2小时或更晚制备的提取物不再转录RNA聚合酶II模板，但保留转录RNA聚合酶III模板以及牛痘病毒DNA的能力。这些深刻的变化，在转录特异性可能有助于选择性表达病毒基因牛痘感染。
A soluble system that specifically and accurately initiates transcription on defined vaccinia virus templates has been obtained from lysates of infected cells. The required regulatory signals are contained within a DNA segment extending about 230 bp upstream and 30 bp downstream of the RNA start site. Transcription is resistant to a-amanitin and inhibited by antibodies to the viral RNA polymerase. Whole cell extracts from uninfected cells cannot accurately transcribe vaccinia DNA. Conversely, extracts prepared at 2 hr or later after vaccinia infection no longer transcribe RNA polymerase II templates but retain the ability to transcribe RNA polymerase Ill templates as well as vaccinia virus DNA. These profound changes in transcriptional specificity may contribute to the selective expression of viral genes following vaccinia infection.