Anabolic signaling and protein synthesis in human skeletal muscle after dynamic shortening or lengthening exercise
Anabolic signaling and protein synthesis in human skeletal muscle after dynamic shortening or lengthening exercise
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DOI：
10.1152/ajpendo.00415.2005
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发表时间：
2006-04-01
影响因子：
5.1
通讯作者：
Rennie, M
中科院分区：
文献类型：
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作者：
Cuthbertson, DJ;Babraj, J;Rennie, M
我们假设在动态缩短或延长运动后，合成代谢信号蛋白质蛋白激酶B（PK B）和p70 S6激酶（p70（S6 K））的差异激活以及随后的人类肌肉蛋白质合成（MPS）的差异刺激。8名健康男性[ 25 +/- 5岁，BMI 26 +/- 3 kg/m（-2）（平均值+/- SD）]在12分钟重复升高至膝盖高度并再次下降之前和之后进行了研究，同时携带其体重的25%，即，用“上”腿进行缩短运动，用对侧“下”腿进行延长运动。在运动前和运动后3、6和24 h进行股四头肌活检。运动后，在活检前2小时，受试者摄入500 ml含有45 g必需氨基酸和135 g蔗糖的水。肌肉蛋白质合成速率通过[1-C-13]亮氨酸（
We hypothesized a differential activation of the anabolic signaling proteins protein kinase B (PKB) and p70 S6 kinase (p70(S6K)) and subsequent differential stimulation of human muscle protein synthesis ( MPS) after dynamic shortening or lengthening exercise. Eight healthy men [ 25 +/- 5 yr, BMI 26 +/- 3 kg/m(-2) ( means +/- SD)] were studied before and after 12 min of repeated stepping up to knee height, and down again, while carrying 25% of their body weight, i.e., shortening exercise with the "up" leg and lengthening exercise with contralateral "down" leg. Quadriceps biopsies were taken before and 3, 6, and 24 h after exercise. After exercise, over 2 h before the biopsies, the subjects ingested 500 ml of water containing 45 g of essential amino acids and 135 g of sucrose. Rates of muscle protein synthesis were determined via incorporation over time of [1-C-13] leucine (