Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification.
Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification.
复制标题
DOI：
10.1182/blood.v78.9.2222.bloodjournal7892222
复制
发表时间：
1991-11
期刊：
影响因子：
20.3
通讯作者：
T. Yi;John L. Cleveland;J. N. Ihle
中科院分区：
文献类型：
--
作者：
T. Yi;John L. Cleveland;J. N. Ihle
聚合酶链反应（PCR）条件下扩增cDNA编码推定的蛋白酪氨酸磷酸酶（PTP）从鼠白细胞介素-3依赖性骨髓细胞系。用于反应的引物基于在所有已知PTP中共享的催化结构域的保守序列。对100个PCR扩增的cDNA克隆进行测序，鉴定出7个不同的cDNA序列。其中两个序列与鼠PTP基因Ly 5/CD 45/LCA和LRP/R-PTP-α相同。其中两个cDNA序列分别与人PTP β（HPTP β）和大鼠脑PTP（PTP 1B）有95%的相同性，可能代表它们的鼠同源物。三个cDNA序列编码新的潜在的PTP。其中一种假定的PTP广泛表达，而另一种主要在脑、肾和肝中表达，在多种其他细胞和组织中表达水平低得多。第三种新的推定磷酸酶主要在造血细胞和组织中表达，其模式与Ly 5/CD 45/LCA相当。对这些新型PTPs的进一步表征将为造血细胞的生长调节提供深入见解。
Polymerase chain reaction (PCR) conditions were used to amplify cDNAs that encode putative protein tyrosine phosphatases (PTPs) from a murine interleukin-3-dependent myeloid cell line. Primers for the reactions were based on conserved sequences of the catalytic domain that are shared among all known PTPs. Sequencing of 100 PCR-amplified cDNA clones identified seven different cDNA sequences. Two of these sequences were identical to the murine PTP genes Ly5/CD45/LCA and LRP/R-PTP-alpha. Two of the cDNA sequences were 95% identical to human PTP epsilon (HPTP epsilon) and rat brain PTP (PTP1B), respectively, and are likely to represent their murine homologs. Three of the cDNA sequences encoded novel potential PTPs. One of the putative PTPs was ubiquitously expressed while a second was predominantly expressed in brain, kidney, and liver and at much lower levels in a variety of other cell tkpes and tissues. The third novel putative phosphatase was expressed primarily in hematopoietic cells and tissues in a pattern that was comparable with Ly5/CD45/LCA. Further characterization of these novel PTPs will provide insights into the growth regulation of hematopoietic cells.