Requirement of S-pombe exonuclease II, a homologue of S-cerevisiae Sep1, for normal mitotic growth and viability
Requirement of S-pombe exonuclease II, a homologue of S-cerevisiae Sep1, for normal mitotic growth and viability
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DOI：
10.1007/s002940050134
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发表时间：
1996-09-01
期刊：
影响因子：
2.5
通讯作者：
Smith, GR
中科院分区：
文献类型：
--
作者：
Szankasi, P;Smith, GR
来自粟酒裂殖酵母的核酸外切酶II（ExoII）是一种5 '->3'单链DNA核酸外切酶。我们已经克隆了它的基因exo 2，其核苷酸序列显示ExoII是多功能酿酒酵母Sep 1蛋白（也称为Kem 1，Xrn 1，Rar 5，Dst 2）的同源物。S.粟酒裂殖酵母exo 2无效突变体对生长是冷敏感的，在限制性温度下具有增加的细胞大小，对有丝分裂抑制剂噻菌灵和咖啡因高度敏感，并且在稳定期迅速死亡。这些表型中的许多与S.啤酒。相比之下，exo 2突变对减数分裂进程只有中等影响，对减数分裂重组没有显著影响。我们讨论了多功能ExoII蛋白的可能功能。
Exonuclease II (ExoII) from Schizosaccharomyces pombe is a 5'-->3' single-stranded DNA exonuclease. We have cloned its gene, exo2, whose nucleotide sequence revealed that ExoII is a homologue of the multifunctional Saccharomyces cerevisiae Sep1 protein (also called Kem1, Xrn1, Rar5, Dst2). S. pombe exo2 null mutants were cold-sensitive for growth, had increased cell size at the restrictive temperature, were hypersensitive to the mitotic inhibitor thiabendazol and to caffeine, and died rapidly in stationary phase. Many of these phenotypes are similar to those of sep1 (kem1 or xrn1) mutants of S. cerevisiae. In contrast, the exo2 mutation had only a moderate effect on progression through meiosis and no significant effect on meiotic recombination. We discuss possible functions of the multifunctional ExoII protein.