Roles of the sodium-translocating NADH:quinone oxidoreductase (Na+-NQR) on vibrio cholerae metabolism, motility and osmotic stress resistance.
Roles of the sodium-translocating NADH:quinone oxidoreductase (Na+-NQR) on vibrio cholerae metabolism, motility and osmotic stress resistance.
复制标题
DOI：
10.1371/journal.pone.0097083
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Häse CC
中科院分区：
文献类型：
--
作者：
Minato Y;Fassio SR;Kirkwood JS;Halang P;Quinn MJ;Faulkner WJ;Aagesen AM;Steuber J;Stevens JF;Häse CC
Na ~+转运型NADH：醌氧化还原酶（Na ~+-NQR）是一种独特的呼吸酶，催化电子从NADH转移到醌，并伴随钠离子跨膜转运。通常，弧菌属，包括霍乱弧菌，具有这种酶，但缺乏质子泵NADH：泛醌氧化还原酶（复合物I）。因此，Na+-NQR应显著有助于胆总管弧菌生理学的多个方面;然而，迄今为止尚未报道该方面的详细表征。在这项研究中，我们广泛调查的Na+-NQR的损失对霍乱弧菌生理的影响，通过使用表型微阵列（Biolog），转录组学和代谢组学分析。表型芯片检测发现霍乱弧菌ΔnqrA-F突变株存在多种代谢缺陷。转录组分析显示，霍乱弧菌ΔnqrA-F突变体在生长早期和中期上调了31个基因，下调了55个基因。上调最多的基因包括cadA和cadB基因，分别编码赖氨酸脱羧酶和赖氨酸/尸胺反向转运蛋白。代谢组学分析进一步表明CadAB活性增加。下调的基因包括唾液酸催化酶基因。代谢组学分析还表明，在霍乱弧菌ΔnqrA-F突变体中，TCA循环的还原途径增加，嘌呤代谢减少。Na+-NQR的缺乏不影响胆固醇弧菌的任何Na+泵相关表型，表明其他次级Na+泵可以补偿Na+-NQR的Na+泵活性。总的来说，我们的研究提供了重要的见解Na+-NQR的贡献V. cholesterol生理。
The Na+ translocating NADH:quinone oxidoreductase (Na+-NQR) is a unique respiratory enzyme catalyzing the electron transfer from NADH to quinone coupled with the translocation of sodium ions across the membrane. Typically, Vibrio spp., including Vibrio cholerae, have this enzyme but lack the proton-pumping NADH:ubiquinone oxidoreductase (Complex I). Thus, Na+-NQR should significantly contribute to multiple aspects of V. cholerae physiology; however, no detailed characterization of this aspect has been reported so far. In this study, we broadly investigated the effects of loss of Na+-NQR on V. cholerae physiology by using Phenotype Microarray (Biolog), transcriptome and metabolomics analyses. We found that the V. cholerae ΔnqrA-F mutant showed multiple defects in metabolism detected by Phenotype Microarray. Transcriptome analysis revealed that the V. cholerae ΔnqrA-F mutant up-regulates 31 genes and down-regulates 55 genes in both early and mid-growth phases. The most up-regulated genes included the cadA and cadB genes, encoding a lysine decarboxylase and a lysine/cadaverine antiporter, respectively. Increased CadAB activity was further suggested by the metabolomics analysis. The down-regulated genes include sialic acid catabolism genes. Metabolomic analysis also suggested increased reductive pathway of TCA cycle and decreased purine metabolism in the V. cholerae ΔnqrA-F mutant. Lack of Na+-NQR did not affect any of the Na+ pumping-related phenotypes of V. cholerae suggesting that other secondary Na+ pump(s) can compensate for Na+ pumping activity of Na+-NQR. Overall, our study provides important insights into the contribution of Na+-NQR to V. cholerae physiology.
登录
查看更多内容
影响因子：
3.2
作者：
Kovacikova, Gabriela;Lin, Wei;Skorupski, Karen
通讯作者：
Skorupski, Karen
影响因子：
3.8
作者：
Minato, Yusuke;Fassio, Sara R.;Haese, Claudia C.
通讯作者：
Haese, Claudia C.
影响因子：
2.9
作者：
Barquera, B;Hellwig, P;Gennis, RB
通讯作者：
Gennis, RB
影响因子：
4.8
作者：
Kirkwood, Jay S.;Legette, LeeCole L.;Stevens, Jan F.
通讯作者：
Stevens, Jan F.
影响因子：
3.1
作者：
Almagro-Moreno, Salvador;Boyd, E. Fidelma
通讯作者：
Boyd, E. Fidelma