Intrinsic lymphotoxin-β receptor requirement for homeostasis of lymphoid tissue dendritic cells
Intrinsic lymphotoxin-β receptor requirement for homeostasis of lymphoid tissue dendritic cells
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DOI：
10.1016/j.immuni.2005.02.007
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发表时间：
2005-04-01
期刊：
影响因子：
32.4
通讯作者：
Cyster, JG
中科院分区：
文献类型：
--
作者：
Kabashima, K;Banks, TA;Cyster, JG
调节树突状细胞（DC）发育和稳态的因素尚不完全理解。在这里，我们证明了DCS表达淋巴毒素（LT）-Beta受体（LTβR），并且在造血细胞中缺乏LTβR的小鼠中，脾脏，脾和淋巴结，CD8（ - ）DC数量减少了。 B细胞是脾脏DC稳态的LT Alpha 1β2的关键来源，B细胞上LT Alpha 1β2的转基因过表达导致CD8-DC隔室的扩展。此外，我们发现约有5％的脾脏DC正在接受细胞分裂，并且在没有LTβ的情况下，分裂的CD8-DC的数量不成比例地减少。在抛物性betaissis实验中，脾脏DC仅通过在循环前体而替换了脾脏DC。一个6周的时间。我们得出的结论是，LT Alpha 1 Beta 2作用于DC或DC前体以促进DC稳态，我们建议DC增殖是将这些细胞局部保持在稳态状态的重要途径。
The factors regulating dendritic cell (DC) development and homeostasis are incompletely understood. Here, we demonstrate that DCs express the lymphotoxin (LT)-beta receptor (LT beta R) and that in mice lacking the LT beta R in hematopoietic cells, spleen, and lymph node, CD8(-) DC numbers are reduced. B cells are a key source of LT alpha 1 beta 2 for splenic DC homeostasis, and transgenic overexpression of LT alpha 1 beta 2 on B cells leads to expansion of the CD8- DC compartment. Furthermore, we find that about 5% of splenic DCs are undergoing cell division, and the number of dividing CD8- DCs is disproportionately reduced in the absence of the LT beta R. In parabiosis experiments, splenic DCs were only partially replaced by circulating precursors over a 6 week period. We conclude that LT alpha 1 beta 2 acts on DCs or DC precursors to promote DC homeostasis, and we suggest that DC proliferation is an important pathway for locally maintaining these cells in the steady state.