PRIMARY SEQUENCE INFORMATION FROM INTACT PROTEINS BY ELECTROSPRAY IONIZATION TANDEM MASS-SPECTROMETRY
PRIMARY SEQUENCE INFORMATION FROM INTACT PROTEINS BY ELECTROSPRAY IONIZATION TANDEM MASS-SPECTROMETRY
复制标题
DOI：
10.1126/science.2326633
复制
发表时间：
1990-04-13
期刊：
影响因子：
56.9
通讯作者：
SMITH, RD
中科院分区：
文献类型：
--
作者：
LOO, JA;EDMONDS, CG;SMITH, RD
串联质谱法已被用于获得完整蛋白质牛核糖核酸酶a的初级序列部分的相关信息。电喷雾电离产生的多个带电分子离子，在三重四极杆质谱仪中以低能碰撞解离，产生单个和多个带电片段离子，可分配给蛋白质的已知序列。高电荷分子离子的解离产生成对的互补产物离子。高阶（气相）蛋白质结构影响解离过程，正如在天然核糖核酸酶A和二硫还原形式的串联质谱比较中观察到的那样。
Tandem mass spectrometry has been used to obtain information related to portions of the primary sequence for an intact protein, bovine ribonuclease A. Multiply charged molecular ions, generated by electrospray ionization, were collisionally dissociated at low energies in a triple quadrupole mass spectrometer to yield single and multiply charged fragment ions that can be assigned to the known sequence of the protein. Dissociation of the highly charged molecular ions resulted in pairs of complementary product ions. The higher order (gas-phase) protein structure affects the dissociation processes, as observed in comparisons of tandem mass spectra of the native and disulfide-reduced forms of ribonuclease A.