Genomic structure and evolutionary conservation of the tyrosinase gene family from Fugu
Genomic structure and evolutionary conservation of the tyrosinase gene family from Fugu
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DOI：
10.1016/s0378-1119(02)00411-0
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发表时间：
2002-02-20
期刊：
影响因子：
3.5
通讯作者：
Beermann, F
中科院分区：
文献类型：
--
作者：
Camacho-H端bner, A;Richard, C;Beermann, F
酪氨酸酶基因家族包括三个成员，酪氨酸酶、酪氨酸酶相关蛋白1（Tyrp 1）和多巴色素互变异构酶（Dct），其编码涉及黑色素合成的蛋白质。在人类和小鼠中，已知所有三种基因的基因组组织，揭示了调控元件和外显子/内含子结构的共同特征。我们已经着手从一种更原始的脊椎动物中鉴定出完整的家族，河豚河豚（Takifugu rubripes），其特征在于紧凑的基因组。我们最近分离并鉴定了河豚酪氨酸酶基因（Genesis 28（2000）99-105）。我们现在报告的其他两个家庭成员，Tyrp 1和Dct的分离和表征。来自这些基因的调控序列在小鼠色素细胞中起作用，并且能够介导报告基因的表达。我们的研究结果表明，存在的所有三个酪氨酸酶家族成员的硬骨鱼，并强调了进化保守的piglitaxel系统。(C)2002 Elsevier Science B. V.保留所有权利。
The tyrosinase gene family encompasses three members, tryosinase, tyrosinase-related protein 1 (Tyrp1) and dopachrome tautomerase (Dct), which encode for proteins implicated in melanin synthesis. In human and mouse, genomic organization is known for all three genes, revealing common features of regulatory elements and of exon/intron structure. We have set out to identify the complete family from a more primitive vertebrate, the pufferfish Fugu (Takifugu rubripes), which is characterized by a compact genome. We had recently isolated and characterized the Fugu tyrosinase gene (Genesis 28 (2000) 99-105). We now report the isolation and characterization of the two other members of the family, Tyrp1 and Dct. Regulatory sequences from these genes function in mouse pigment cells and are able to mediate reporter gene expression. Our results demonstrate the existence of all three tyrosinase family members in teleosts and underline the evolutionary conservation of the pigmentary system. (C) 2002 Elsevier Science B.V. All rights reserved.