Identification of urinary tellurium metabolite in rats administered sodium tellurite
Identification of urinary tellurium metabolite in rats administered sodium tellurite
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DOI：
10.1039/b613835g
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发表时间：
2007-02
影响因子：
3.4
通讯作者：
Y. Ogra;Reina Kobayashi;K. Ishiwata;Kazuo T. Suzuki
中科院分区：
文献类型：
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作者：
Y. Ogra;Reina Kobayashi;K. Ishiwata;Kazuo T. Suzuki
碲（Te）是重要的工业类金属之一，广泛存在于环境中。然而，尽管碲被认为是一种有害元素，但其生物学和毒理学效应仍不清楚。在这项研究中，我们试图通过HPLC-ICP-MS和电喷雾电离（ESI）-MS鉴定大鼠尿中的Te代谢物（UTM）。为了明确鉴定UTM，使用了两种不同的色谱机制，即，使用多模式凝胶过滤和阳离子交换柱。摄入亚碲酸盐后检测到的主要UTM是三甲基碲，尽管主要的尿硒代代谢物是硒糖（甲基-2-乙酰氨基-2-脱氧-1-硒代-β-D-吡喃半乳糖苷），但未检测到含Te的尿糖代谢物。有趣的是，亚碲酸盐的摄入增强了尿中硒代蛋氨酸的排泄。这些结果表明，碲是离散代谢的硒（Se），属于同一组的必需元素，虽然它影响硒在大鼠体内的代谢。因此，硒代谢障碍，即，硒缺乏可能是碲的潜在毒性作用之一。
Tellurium (Te) is one of the important metalloids used in industry, and is ubiquitously found in the environment. However, the biological and toxicological effects of Te are still unclear despite its being recognized as a hazardous element. In this study, we attempted to identify urinary Te metabolites (UTMs) in rats by HPLC-ICP-MS and electrospray ionization (ESI)-MS. To unambiguously identify UTMs, two different chromatographic mechanisms, i.e., multi-mode gel filtration and cation exchange columns, were employed. The major UTM detected after ingestion of tellurite was trimethyltelluronium, and no urinary sugar metabolites containing Te were detected despite the fact that the major urinary selenometabolite was a selenosugar (methyl-2-acetamido-2-deoxy-1-seleno-β-D-galactopyranoside). Interestingly, the ingestion of tellurite enhanced the excretion of selenometabolites in urine. These results suggest that Te is discretely metabolized from selenium (Se), an essential element belonging to the same group, although it affects the metabolism of Se in rats. Thus, the disturbance of Se metabolism, i.e., the induction of Se deficiency, may be one of the potential toxic effects of Te.