Supplementation with tomato-based products increases lycopene, phytofluene, and phytoene levels in human serum and protects against UV-light-induced erythema
Supplementation with tomato-based products increases lycopene, phytofluene, and phytoene levels in human serum and protects against UV-light-induced erythema
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DOI：
10.1024/0300-9831.75.1.54
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发表时间：
2005-01-01
影响因子：
2.3
通讯作者：
Heinrich, U
中科院分区：
文献类型：
--
作者：
Aust, O;Stahl, W;Heinrich, U
类胡萝卜素是合适的光保护剂，而β-胡萝卜素补充剂用于防止紫外线(UV)光诱导的红斑。当饮食中提供类胡萝卜素时，也可以观察到保护作用。在这里，我们研究了合成番茄红素的光保护作用，并与番茄提取物(Lyc-o-Mato(R))和含有增溶Lyc-o-Mato(R)的饮料(Lyc-o-Guard饮料)进行了比较。对于这些不同的来源，志愿者摄入了类似数量的番茄红素(约10毫克/天)。补充12周后，所有组的番茄红素血清水平和总皮肤类胡萝卜素均显著增加。在Lyc-o-Mato组和Lyc-o-Guard饮料组中，血清植物荧烯和植物烯水平显著升高。在0、4和12周，用太阳光模拟器诱发红斑。用1.25最小红斑量(MED)照射受试者背部皮肤。在照射前和照射后24小时用比色法评估皮肤发红情况，并以阳性a值(红/绿轴)表示。以a值(照射前和照射后24小时的差值)作为红斑强度的指标。从0周到12周，所有组的Delta a值都有所下降，这表明红斑的形成得到了预防。与0周相比，合成番茄红素组在12周时的增量a值降低了25%。这种保护作用在Lyc-o-Mato(38%)和Lyc-o-Guard-Drink(48%)组中更为明显。在后两组中，植氟烯和植烯可能起到了保护作用。这两种类胡萝卜素在紫外光的波长都有最大吸收。紫外线的吸收保护皮肤免受光损伤，这可能解释了观察到的不同组之间的差异。
Carotenoids are suitable photoprotectants, and beta-carotene supplements are used for protection against ultraviolet (UV) light-induced erythema. Protective effects are also observed when carotenoids are provided with the diet. Here, we investigated the photoprotective effects of synthetic lycopene in comparison with a tomato extract (Lyc-o-Mato (R)) and a drink containing solubilized Lyc-o-Mato (R) (Lyc-o-Guard-Drink). With these different sources, the volunteers ingested similar amounts of lycopene (about 10 mg/day). After 12 weeks of supplementation, significant increases in lycopene serum levels and total skin carotenoids were observed in all groups. Significant increases in the serum levels of phytofluene and phytoene occurred in the Lyc-o-Mato and the Lyc-o-Guard-Drink group.At weeks 0, 4, and 12 an erythema was induced with a solar light simulator. Dorsal skin of each subject was irradiated with 1.25 minimal erythemal dose (MED). Reddening of the skin was evaluated before and 24 hours after irradiation by chromametry and expressed as positive a-values (red/green-axis). Delta a-values (difference of a-value before irradiation and after 24 hours) were used as an index of erythema intensity. A decrease in the Delta a-value from week 0 to week 12, indicating prevention of erythema formation, was observed in all groups. Compared to week 0, the Delta a-value at week 12 was 25% lower in the synthetic lycopene group. The protective effect was more pronounced in the Lyc-o-Mato (38%) and Lyc-o-Guard-Drink (48%) groups. In the two latter groups, phytofluene and phytoene may have contributed to protection. Both of these carotenoids exhibit absorption maxima at wavelengths of UV light. Absorption of UV light protects skin from photodamage and might explain the differences observed between groups.