Insulin and leptin enhance human sperm motility, acrosome reaction and nitric oxide production
Insulin and leptin enhance human sperm motility, acrosome reaction and nitric oxide production
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DOI：
10.1111/j.1745-7262.2008.00421.x
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发表时间：
2008-09-01
影响因子：
2.9
通讯作者：
du Plessis, Stefan S.
中科院分区：
文献类型：
--
作者：
Lampiao, Fanuel;du Plessis, Stefan S.
目的：研究胰岛素和瘦素对人精子运动力、活力、顶体反应和一氧化氮（NO）生成的影响。方法：用胰岛素（10 μ IU）和瘦素（10 nmol）处理正常精子供体的洗涤精子。胰岛素和瘦素的作用被阻断抑制其细胞内效应，磷脂酰肌醇3-激酶（PI 3 K），渥曼青霉素（10 μ mol）30分钟前胰岛素和瘦素被给予。在孵育1、2和3 h后，使用计算机辅助精液分析来评估活力。通过使用碘化丙啶作为荧光探针的荧光激活细胞分选来评估活力。以荧光素-异硫氰酸酯-豌豆凝集素为探针，在荧光显微镜下观察顶体反应细胞。用4，5-二氨基荧光素-2/二乙酸酯（4，5-diaminofluorescein-2/diacetate，DA 2/DA）处理精子后测定NO，并通过荧光激活细胞分选术进行分析。结果：胰岛素和瘦素均能显著增加心肌细胞的总运动、前向运动和顶体反应，并能增加NO的产生。结论：胰岛素和瘦素在体外对人精子功能有促进作用。这些激素可能在增强人类精子的受精能力方面发挥作用。
Aim: To investigate the in vitro effects of insulin and leptin on human sperm motility, viability, acrosome reaction and nitric oxide (NO) production. Methods: Washed human spermatozoa from normozoospermic donors were treated with insulin (10 mu IU) and leptin (10 nmol). Insulin and leptin effects were blocked by inhibition of their intracellular effector, phosphotidylinositol 3-kinase (PI3K), by wortmannin (10 mu mol) 30 min prior to insulin and leptin being given. Computer-assisted semen analysis was used to assess motility after 1, 2 and 3 h of incubation. Viability was assessed by fluorescence-activated cell sorting using propidium iodide as a fluorescent probe. Acrosome-reacted cells were observed under a fluorescent microscope using fluorescein-isothiocyanate-Pisum sativum agglutinin as a probe. NO was measured after treating the sperm with 4,5-diaminofluorescein-2/diacetate (DAF-2/DA) and analyzed by fluorescence-activated cell sorting. Results: Insulin and leptin significantly increased total motility, progressive motility and acrosome reaction, as well as NO production. Conclusion: This study showed the in vitro beneficial effects of insulin and leptin on human sperm function. These hormones could play a role in enhancing the fertilization capacity of human spermatozoa.