THE RELEVANCE OF FLOW-CYTOMETRIC DNA CONTENT IN THE EVALUATION OF LUNG-CANCER
THE RELEVANCE OF FLOW-CYTOMETRIC DNA CONTENT IN THE EVALUATION OF LUNG-CANCER
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DOI：
10.1007/bf01372562
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发表时间：
1994-02-01
影响因子：
3.6
通讯作者：
GOHDE, W
中科院分区：
文献类型：
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作者：
SALVATI, F;TEODORI, L;GOHDE, W
用流式细胞术研究了185例恶性肿瘤患者（160例非小细胞肺癌，13例小细胞肺癌和12例非上皮性肿瘤）和6例良性肺肿瘤患者的细胞，以检测DNA含量的预后价值。A.非小细胞肺癌（NSCLC）144例（90%），小细胞肺癌（SCLC）8例（62%）。此外，52%（83例患者）的NSCLC、24%（3例患者）的SCLC和50%（6例患者）的非上皮性肿瘤表现出多克隆性。良性肿瘤呈二倍体DNA含量。使用Bergesson和Gage方法和Greenwood方差进行精算生存分析，选择了142例患者。通过使用t检验对固定时间之间的未配对数据进行统计学比较。未观察到倍性与分期、组织学分级或治疗方式之间的相关性。随访12、18和24个月后，观察到二倍体和单克隆（排除亚和超四倍体）患者的生存率在统计学上显著更好。因此，流式细胞术DNA分析可能是有用的人肺肿瘤的预后评估。
Cells from a group of 185 patients suffering from malignant tumours (160 non-small-cell lung carcinoma, 13 small-cell lung carcinoma, and 12 non-epithelial tumours) and 6 with benign lung tumours were studied by flow cytometry in order to detect the prognostic value of DNA content. A. total of 144 (90%) non-small-cell lung carcinomas (NSCLC) and 8 (62%) small-cell lung carcinomas (SCLC) exhibited aneuploidy. Furthermore 52% (83 patients) NSCLC, 24% (3 patients) SCLC and 50% (6 patients) nonepithelial tumours demonstrated multiclonality. Benign cases showed diploid DNA content. For actuarial survival analysis using the Bergesson and Gage method and the Greenwood variance, 142 patients were selected. Statistical comparisons were made by the use of the t-test for unpaired data between fixed times. No correlation was observed between ploidy and stage, histological grading or treatment modality. A statistically significantly better survival was observed after 12, 18 and 24 months of follow-up for diploid and monoclonal (with the exclusion of hypo- and hypertetraploid) patients. Thus, flow-cytometric DNA analysis may be useful in prognostic assessment of human lung tumours.