Evaluation of monkeypox virus infection of black-tailed prairie dogs (Cynomys ludovicianus) using in vivo bioluminescent imaging.
Evaluation of monkeypox virus infection of black-tailed prairie dogs (Cynomys ludovicianus) using in vivo bioluminescent imaging.
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DOI：
10.7589/2013-07-171
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发表时间：
2014-07
影响因子：
1.3
通讯作者：
Rocke TE
中科院分区：
文献类型：
--
作者：
Falendysz EA;Londoño-Navas AM;Meteyer CU;Pussini N;Lopera JG;Osorio JE;Rocke TE
猴痘 (MPX) 是一种重新出现的人畜共患疾病，在中非和西非流行，可在人类中引起类似天花的疾病。尽管对 MPX 进行了许多流行病学和现场调查，但尚未确定明确的储存宿主物种。使用表达萤火虫荧光素酶 (luc) 基因的重组病毒，我们之前证明了体内生物发光成像 (BLI) 在动物模型中研究 MPX 发病机制的适用性。在这里，我们评估了 BLI 作为追踪黑尾草原土拨鼠 (Cynomys ludovicianus) 中 MPX 病毒感染的新方法。 2003 年美国多州爆发 MPX 期间，草原土拨鼠受到影响，此后一直被用作该疾病的动物模型。我们的 BLI 结果与 PCR 和从死后收集的组织中分离的病毒进行了比较。在临床前和临床阶段期间以及亚临床继发病例中，通过 BLI 可以轻松地在皮肤和浅表组织中检测到病毒并对其进行定量，但在肺部等深层组织中却无法可靠地检测到病毒。尽管大型野生啮齿动物中的病毒检测存在局限性，但 BLI 可以增强草原土拨鼠作为 MPX 动物模型的使用，并可用于研究潜在野生啮齿动物宿主的感染、疾病进展和传播。
Monkeypox (MPX) is a re-emerging zoonotic disease that is endemic in Central and West Africa, where it can cause a smallpox-like disease in humans. Despite many epidemiologic and field investigations of MPX, no definitive reservoir species has been identified. Using recombinant viruses expressing the firefly luciferase (luc) gene, we previously demonstrated the suitability of in vivo bioluminescent imaging (BLI) to study the pathogenesis of MPX in animal models. Here, we evaluated BLI as a novel approach for tracking MPX virus infection in black-tailed prairie dogs (Cynomys ludovicianus). Prairie dogs were affected during a multi-state outbreak of MPX in the USA in 2003 and have since been used as an animal model of this disease. Our BLI results were compared with PCR and virus isolation from tissues collected postmortem. Virus was easily detected and quantified in skin and superficial tissues by BLI before and during clinical phases, as well as in subclinical secondary cases, but was not reliably detected in deep tissues such as the lung. Although there are limitations to viral detection in larger wild rodent species, BLI can enhance the use of prairie dogs as an animal model of MPX and can be used for the study of infection, disease progression, and transmission in potential wild rodent reservoirs.