Use of spray-dried zirconia microspheres in the separation of immunoglobulins from cell culture supernatant.
Use of spray-dried zirconia microspheres in the separation of immunoglobulins from cell culture supernatant.
复制标题
使用喷雾干燥的氧化锆微球从细胞培养物上清液中分离免疫球蛋白。
DOI：
10.1016/s0021-9673(00)00289-2
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
McNeff,CV
中科院分区：
文献类型：
--
作者：
Subramanian,A;Carr,PW;McNeff,CV
A method suitable for the isolation of monoclonal antibodies (MAbs) on novel zirconia microspheres (20–30 μm) is described. Zirconia microspheres were generated by spray drying colloidal zirconia. Spray-dried zirconia microspheres were further classified and characterized by X-ray diffraction, BET porosimetry and scanning electron microscopy. Spray-dried zirconia microspheres were modified with ethylenediamine-N,N′-tetra(methylenephosphonic) acid (EDTPA) to create a cation-exchange chromatographic support. The chromatographic behavior of a semi-preparative column packed with EDTPA-modified zirconia microspheres was evaluated and implications for scale-up are provided. EDTPA-modified zirconia microspheres were further used to purify MAbs from cell culture supernatant. Analysis by enzyme linked immunosorbent assay and gel electrophoresis demonstrate that MAbs can be recovered from a cell culture supernatant at high yield (92–98%) and high purity (>95%) in a single chromatographic step.
