Prolactin of normal and dwarf mice.
Prolactin of normal and dwarf mice.
复制标题
正常和侏儒小鼠的催乳素。
DOI：
10.1210/endo-85-4-698
复制
发表时间：
1969
期刊：
影响因子：
4.8
通讯作者：
U. Lewis
中科院分区：
文献类型：
--
作者：
E. Cheever;B. K. Seavey;U. Lewis
小鼠的催乳素和白蛋白，无论是作为两种纯化蛋白的混合物分析，还是作为脑垂体匀浆的成分分析，都不能用pH 9.5的圆盘电泳法很好地分离，除非丙烯酰胺浓度超过7.5%。用10%的丙烯酰胺制成的凝胶，获得了所有主要电泳谱的最大分辨率，然后可以用这个程序来显示不同条件下小鼠脑垂体催乳素带中蛋白质含量的差异。雌鼠脑下垂体中该带的数量比雄鼠多，雌鼠注射雌二醇后，雄鼠脑下垂体中该带的数量增加。用制备电泳法分离得到一条具有催乳素活性的蛋白条带，经鸽粮囊试验鉴定为具有催乳素活性。测得其相对分子质量为23000±1000。
Prolactin and albumin of the mouse, when analyzed either as a mixture of the two purified proteins or as constituents of a pituitary homogenate, were not well separated from each other by disc-electrophoresis at pH 9.5 unless the concentration of acrylamide exceeded 7.5%. By using a gel made with 10% acrylamide, a maximum resolution of all the major components of the electrophoretic pattern was obtained, and the procedure then could be used to demonstrate differences in the amount of protein in the prolactin band in the pituitary gland of the mouse under various conditions. Both the greater amount of this band in the pituitary gland of the female mouse as compared to the male, and its increase in the pituitary of the male after administration of estradiol were detected easily. The band designated as prolactin in the electrophoretic pattern was isolated by preparative electrophoresis and identified as having prolactin activity by the pigeon crop sac assay. A molecular weight of 23,000 ± 1000 was found for ...