Prevention of reperfusion injury of the spinal cord in aortic surgery:: An experimental study
Prevention of reperfusion injury of the spinal cord in aortic surgery:: An experimental study
复制标题
DOI：
10.1007/s00595-007-3614-5
复制
发表时间：
2008-03-01
期刊：
影响因子：
2.5
通讯作者：
Kocak, Hikmet
中科院分区：
文献类型：
--
作者：
Kocogullari, Cevdet Ugur;Becit, Necip;Kocak, Hikmet
目的.我们设计了一项实验研究，以显示一些药物的作用，以防止再灌注损伤的脊髓。本实验选用家兔20只，随机分为两组。第1组受试者仅在肾动脉和髂动脉分叉之间施行肾下腹主动脉闭塞30分钟;另一方面，第2组受试者在肾下腹主动脉闭塞后施行主动脉内地尔硫卓、N-乙酰半胱氨酸和过氧化氢酶联合给药。术后48 h采用Tarlov评分法评价脊髓功能，并取脊髓组织进行生化和组织病理学检查。第2组患者神经功能评分明显优于第1组（P < 0.01）。第2组;超氧化物歧化酶和谷胱甘肽过氧化物酶水平升高，丙二醛和黄嘌呤氧化酶水平降低（P < 0.05）。组织病理学检查显示第2组样本具有较少的出血点和较少的神经元丢失。我们的结论是，抗氧化剂组合（地尔硫卓，N-乙酰半胱氨酸，和过氧化氢酶）缺血后应用，因此可能有助于保护脊髓缺血和再灌注损伤。
Purpose. We designed an experimental study to show the effects of some agents in order to prevent reperfusion injury of the spinal cord.Methods. Twenty rabbits were used and were divided into two groups in our study. Infrarenal abdominal aortic occlusion, between renal arteries and iliac bifurcations, was applied to the subjects in group 1 for only 30 min; in the group 2 subjects, on the other hand, intra-aortic diltiazem, N-acetylcysteine, and catalase combinations were applied after infrarenal abdominal aortic occlusion. The spinal cord functions of the subjects were assessed at the 48th hour after the operation according to Tarlov scoring, then cord tissue samples were taken for biochemical and histopathological studies.Results. The group 2 subjects had better neurological functions than group 1 subjects (P < 0.01). In group 2; superoxide dismutase and glutathione peroxidase levels increased, while malondialdehyde and xanthine oxidase levels decreased as compared with group 1 (P < 0.05). A histopathological examination showed the group 2 samples to have fewer bleeding points and less neuron loss.Conclusions. We concluded that antioxidant agent combinations (diltiazem, N-acetylcysteine, and catalase) applied after ischemia might thus help protect the spinal cord against ischemia and reperfusion injury.