A gravity-driven droplet fluidic point-of-care test.
A gravity-driven droplet fluidic point-of-care test.
复制标题
DOI：
10.1016/j.device.2023.100009
复制
发表时间：
2023-07
期刊：
影响因子：
--
通讯作者：
H. Vahabi;Jason Liu;Yifan Dai;D. Joh;Rhett J Britton;Jacob T. Heggestad;David S. Kinnamon;Satyam Rajput;Ashutosh Chilkoti
中科院分区：
文献类型：
--
作者：
H. Vahabi;Jason Liu;Yifan Dai;D. Joh;Rhett J Britton;Jacob T. Heggestad;David S. Kinnamon;Satyam Rajput;Ashutosh Chilkoti
我们报告了一个简单的液滴流体即时检测（POCT），它使用重力来操纵表面上液滴的顺序、时间和运动。为了制造这种POCT，我们首先开发了一个表面涂层工具箱，其中包含九种不同的涂层，具有三种润湿性和三种光滑度，可以独立定制。然后，我们制造了一个设备，具有互连的流体元件泵，流阻器和导流板在一个高度光滑的固体表面上，以精确地控制多个液滴的运动的时间和顺序，以及它们在表面上的相互作用。然后，我们使用该设备对临床相关分析物-乳酸脱氢酶（LDH）进行多步酶测定，以证明该技术在床旁诊断中的应用。
We report a simple droplet fluidic point-of-care test (POCT) that uses gravity to manipulate the sequence, timing, and motion of droplets on a surface. To fabricate this POCT, we first developed a surface coating toolbox of nine different coatings with three levels of wettability and three levels of slipperiness that can be independently tailored. We then fabricated a device that has interconnected fluidic elements—pumps, flow resistors, and flow guides—on a highly slippery solid surface to precisely control the timing and sequence of motion of multiple droplets and their interactions on the surface. We then used this device to carry out a multi-step enzymatic assay of a clinically relevant analyte—lactate dehydrogenase (LDH)—to demonstrate the application of this technology for point-of-care diagnosis.