Rapid assays for detection of anti-islet autoantibodies: Implications for organ donor screening
Rapid assays for detection of anti-islet autoantibodies: Implications for organ donor screening
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DOI：
10.1006/jaut.2000.0457
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发表时间：
2001-02-01
影响因子：
12.8
通讯作者：
Eisenbarth, GS
中科院分区：
文献类型：
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作者：
Maniatis, AK;Yu, LP;Eisenbarth, GS
本研究的目的是开发和评估GAD65 （GAA）、ICA512bdc/IA-2 （ICA512AA）和胰岛素（microLAA, mIAA）自身抗体的快速检测方法，作为鉴定糖尿病高风险尸体胰腺供体的潜在工具。该研究包括154名新发糖尿病、糖尿病前期和健康对照者。在三个单独的检测中评估受试者的所有三种自身抗体：(1)标准（std）检测，在4℃孵育24小时或72小时（分别联合GAA/ICA512AA或mIAA），(2)快速检测，室温孵育1小时，(3)快速检测，室温孵育2小时。同时对777例器官供者的血清样本进行了三种自身抗体的检测，并对所有标准检测阳性样本进行了1小时孵育试验。简单的线性回归分析表明，标准法与快速法对三种自身抗体的检测结果具有良好的相关性：(1)GAA: std对1小时（R-2=0.85）和std对2小时（R-2=0.83）， (2) ICA512AA: std对1小时（R-2=0.85）和std对2小时（R-2=0.84）， (3) mIAA: std对Ih （R-2=0.70）和std对2小时（R-2=0.64）。受试者队列间的检测相关率比较显示无显著差异。与各自的标准法相比，1小时RT GAA法漏检3.2%，多检出1.3%的样品；1小时RT ICA512AA法无不一致样品；1小时RT mIAA法漏检7.1%，多检出5.8%的样品。我们分析了储存在尸体捐献者体内的777份血清样本。777例患者中2例（0.25%）自身抗体（GAA和ICA512AA）阳性，23例（3.0%）自身抗体（11 IAA, 12 GAA）阳性。所有三种自身抗体的快速分析可以在不到3小时内完成，其一致性率与更耗时的标准分析相当，使这些分析成为器官供体筛选的一个有吸引力的选择，以确定潜在的胰脏免疫病理研究。(C) 2001学术出版社。
The purpose of the current study was to develop and evaluate rapid assays for autoantibodies to GAD65 (GAA), ICA512bdc/IA-2 (ICA512AA), and insulin (microLAA, mIAA) as a potential tool for identification of cadaveric pancreas donors who were at high risk for developing diabetes. The study included 154 new onset diabetic, prediabetic, and healthy control subjects. Subjects were evaluated for all three autoantibodies in three separate assays: (1) standard (std) assay with a 24-h or 72-h incubation at 4 degreesC (combined GAA/ICA512AA or mIAA, respectively), (2) rapid assay with 1-h room temperature (RT) incubation, and (3) rapid assay with 2-h RT incubation. The serum samples from 777 organ donors were also evaluated for all three autoantibodies and all the positive samples from standard assay evaluated with the 1-h incubation assay. Simple linear regression analyses revealed excellent correlation between the standard assay and the rapid assays for all three autoantibodies, as follows: (1) GAA: std vs. 1 h (R-2=0.85) and std vs. 2 h (R-2=0.83), (2) ICA512AA: std vs. 1 h (R-2=0.85) and std vs. 2 h (R-2=0.84), and (3) mIAA: std vs. Ih (R-2=0.70) and std vs. 2 h (R-2=0.64). Comparison of assay correlation rates between subject cohorts revealed no significant differences. Compared to their respective standard assays, the 1-h RT GAA assay missed 3.2% and identified an additional 1.3% of samples, the 1-h RT ICA512AA assay had no discordant samples, and the 1-h RT mIAA assay missed 7.1% and identified an additional 5.8% of samples. We analysed a series of 777 stored serum samples from cadaveric donors. Two of 777 (0.25%) were positive for two autoantibodies (both GAA and ICA512AA) and 23 of 777 (3.0%) one autoantibody (11 IAA; 12 GAA). The rapid analysis for all three autoantibodies could be completed in less than 3 h with comparable concordance rates to the more time-consuming standard assays, making these assays an attractive option for organ donor screening to identify potential pancreata for immunopathogenetic research. (C) 2001 Academic Press.