The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells
The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells
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人类原始生殖细胞的转录组和 DNA 甲基化组景观
DOI：
10.1016/j.cell.2015.05.015
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发表时间：
2015-06-04
期刊：
影响因子：
64.5
通讯作者：
Qiao, Jie
中科院分区：
文献类型：
--
作者：
Guo, Fan;Yan, Liying;Qiao, Jie
生殖细胞对于遗传信息的代代相传和维持物种的延续至关重要。在这里，我们以单细胞和单碱基分辨率分析了人类原始生殖细胞（PGCs）从迁移期到性腺期的转录组。人类PGCs显示出独特的转录模式，涉及多能基因和种系特异性基因的同时表达，其中一部分显示出发育阶段特异性特征。此外，我们分析了人类PGCs的DNA甲基组，并发现了其基因组的全局去甲基化。大约在妊娠10到11周后，PGCs几乎没有任何DNA甲基化，男性和女性PGCs中甲基化水平分别只有7.8%和6.0%。我们的工作为破译生殖系复杂的表观遗传重编程铺平了道路，目的是恢复受精卵细胞的全能性。
Germ cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental-stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10 to 11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation, with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.