Permanent preparations of stained whole‐body sections using ‘Trycolac‘
Permanent preparations of stained whole‐body sections using ‘Trycolac‘
复制标题
使用“Trycolac”永久制备染色的全身切片
DOI：
10.1111/j.1365-2818.1973.tb03793.x
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发表时间：
1973
影响因子：
2
通讯作者：
Nicholas C. Woods
中科院分区：
文献类型：
--
作者：
D. Farebrother;Nicholas C. Woods
通过Ullberg技术切割的全身切片的永久性制备可以使用“喷雾”封固剂（“Trycolac”）制备，这就不需要载玻片和盖玻片。可使用常规染色方法，前提是染色剂的酒精含量较低。甘油用于脱水和清洁，并喷洒一层“Trycolac”。
Permanent preparations of whole‐body sections cut by Ullberg's technique can be prepared using a ‘spray‐on’ mountant (‘Trycolac’), which does away with the necessity for glass slides and coverslips. Routine staining methods can be used, providing the alcohol content of the stain is low. Glycerol is used for dehydration and clearing and a covering of ‘Trycolac’ is sprayed on.