A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles
A DNA duplex with extremely enhanced thermal stability based on controlled immobilization on gold nanoparticles
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DOI：
10.1021/nl0524748
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发表时间：
2006-03-01
期刊：
影响因子：
10.8
通讯作者：
Sugimoto, N
中科院分区：
文献类型：
--
作者：
Akamatsu, K;Kimura, M;Sugimoto, N
研究了 DNA 负载量对固定在金纳米粒子上的 DNA 双链体热稳定性的影响。与游离双链体（不含金纳米颗粒）相比，金纳米颗粒上适度负载的双链体显示出增强的热稳定性。然而，高负载双链体表现出与游离双链体相似的稳定性。只需改变盐浓度（超过 50 摄氏度）即可在很宽的温度范围内控制稳定性。此外，基于固定双链体热稳定性的提高，具有适度负载的寡核苷酸的金纳米颗粒可以用作纳米探针，用于有效和快速的链交换反应。这些结果表明双链体与纳米颗粒表面之间的相互作用在确定双链体的稳定性中起着重要作用。
The effect of DNA loadings on the thermal stability of DNA duplex immobilized on gold nanoparticles has been investigated. The modestly loaded duplexes on the gold nanoparticles showed enhanced thermal stability, as compared to that of the free duplex (without gold nanoparticles). However, the highly loaded duplex showed stability similar to that of free duplex. The stability could be controlled over a wide temperature range simply by varying the salt concentration (over 50 degrees C). Additionally, the gold nanoparticles with modestly loaded oligonucleotides could be used as nanoprobes for effective and fast strand exchange reactions, based on the increased thermal stability of the immobilized duplex. These results indicate that the interaction between the duplex and the nanoparticle surface plays an important role in determining the stability of the duplex.