Myosin content and myosin isoenzyme distribution in the heterotopic rat heart allograft.
Myosin content and myosin isoenzyme distribution in the heterotopic rat heart allograft.
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异位大鼠同种异体心脏移植物中肌球蛋白含量和肌球蛋白同工酶分布。
DOI：
10.1016/s0022-2828(87)80620-x
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发表时间：
1987
影响因子：
5
通讯作者：
Zerbe,TR
中科院分区：
文献类型：
--
作者：
Klein,I;Hong,C;Zerbe,TR
异位心脏移植是血管灌注的器官，可用于研究心肌蛋白含量的调节。先前的研究表明，同种心脏移植物会出现明显萎缩，重量和肌球蛋白含量下降。在本研究中，我们研究了异位心脏同种异体移植物的大小、肌球蛋白含量和肌球蛋白同工酶分布的变化。移植后六天，同种异体移植心脏不能自发跳动，组织学显示坏死和细胞浸润的证据。与原位心脏相比，同种异体移植心脏的总心脏重量（816±16 mg）和蛋白质含量（117±7 mg）显着更高（分别为471±11和90±5 mg，（P<0.01）。与重量的增加相反，肌球蛋白ATP酶（26％）、肌球蛋白同工酶的V1异构体（43％）和肌球蛋白含量同时减少(53%) 在同种异体移植心脏中，与同种异体移植心脏相似，同种异体移植心脏的肌球蛋白含量减少，肌球蛋白同工酶发生变化，与同种异体移植心脏明显萎缩相反，同种异体移植心脏重量增加很可能是由于细胞浸润排斥反应和水含量增加。
Heterotopic cardiac transplants are vascularly perfused organs that can be used to study the regulation of myocardial protein content. Prior studies have demonstrated that cardiac isografts undergo marked atrophy with a decrease in weight and myosin content. In the present studies we have investigated the changes in size, myosin content and myosin isoenzyme distribution in the heterotopic cardiac allografts. Six days after transplantation allograft hearts were not spontaneously beating and histologically showed evidence of necrosis and cellular infiltration. Total heart weight (816±16 mg) and protein content (117±7 mg) were significantly greater in the allografts compared toin situhearts (471±11 and 90±5 mg respectively, (P<0.01). In contrast to the increase in weight there was a simultaneous decrease in myosin ATPase (26%), theV1isoform of the myosin isoenzyme (43%), and myosin content (53%) in the allograft heart. These studies demonstrate that similar to cardiac isografts, allograft hearts undergo a decrease in myosin content and a shift in myosin isoenzymes. In contrast to the marked atrophy of the cardiac isograft, the allograft heart weight is increased most likely due to rejection with cellular infiltration and an increased water content.