HUMAN-SPECIFIC NUCLEAR-PROTEIN THAT ASSOCIATES WITH THE POLAR REGION OF THE MITOTIC APPARATUS - DISTRIBUTION IN A HUMAN-HAMSTER HYBRID CELL
HUMAN-SPECIFIC NUCLEAR-PROTEIN THAT ASSOCIATES WITH THE POLAR REGION OF THE MITOTIC APPARATUS - DISTRIBUTION IN A HUMAN-HAMSTER HYBRID CELL
复制标题
DOI：
10.1016/0092-8674(80)90359-1
复制
发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
PETTIJOHN, DE
中科院分区：
文献类型：
--
作者：
LYDERSEN, BK;PETTIJOHN, DE
描述了一种在有丝分裂过程中成为有丝分裂器一部分的主要核蛋白。这种蛋白质被称为核有丝分裂器（NuMA）蛋白。在间期细胞中，NuMA蛋白仅限于细胞核，是分离的核基质的组成部分，但在有丝分裂细胞中，通过间接免疫荧光显微镜观察到其集中在有丝分裂器的极区。这种有丝分裂定位取决于纺锤体的完整性，因为破坏纺锤体的处理导致NuMA蛋白在整个细胞中分散。微管蛋白在细胞周期的不同阶段的亚细胞分布的比较表明，NuMA蛋白是不同于先前确定的有丝分裂纺锤体的组件。它与核基质的关联及其在有丝分裂过程中定位到核重组的位点表明了一种有趣的可能性，即NuMA蛋白可能是参与核重组早期事件的一类蛋白质的代表。NuMA存在于已检查的所有类型人类细胞的细胞核和有丝分裂纺锤体中，但在其他种属（包括猴）的细胞中未检测到类似MW（解离溶剂中300，000道尔顿）或免疫特异性的蛋白质。NuMA蛋白在含有减少数量的人染色体的人/中国仓鼠杂交细胞中合成。该杂交细胞的免疫荧光研究表明，NuMA蛋白的分布与人细胞中的分布相同。编码NuMA蛋白的人基因与编码人特异性核蛋白的其他基因不同，可以在人/仓鼠杂交细胞中表达。细胞杂交将有助于进一步表征NuMA蛋白。
A predominantly nuclear protein which becomes part of the mitotic apparatus during mitosis is described. This protein is termed the nuclear-mitotic apparatus (NuMA) protein. In interphase cells NuMA protein is restricted to the nucleus and is a constituent of isolated nuclear matrices, but in mitotic cells it is observed by indirect immunofluorescence microscopy to be concentrated at the polar regions of the mitotic apparatus. This mitotic localization is dependent on the integrity of the spindle, since treatments which disrupt the spindle result in dispersion of NuMA protein throughout the cell. Comparison to the subcellar distribution of tubulin at different stages of the cell cycle indicates that NuMA protein is distinct from the previously identified components of the mitotic spindle. Its association with the nuclear matrix and its localization during mitosis to the site of nuclear reassembly suggest the interesting possibility that NuMA protein could be representative of a class of proteins involved in the early events of nuclear reassembly. NuMA is present in the nuclei and mitotic spindle of all types of human cells that have been examined, but proteins of similar MW (300,000 daltons in dissociating solvents) or immunological specificity are not detected in cells of other species (including monkey). The NuMA protein is synthesized in a human/Chinese hamster hybrid cell containing a reduced number of human chromosomes. Immunofluorescence studies of this hybrid cell showed that the distribution of NuMA protein is equivalent to that in human cells. The human gene coding for NuMA protein, unlike other genes coding for human specific nuclear proteins, can be expressed in human/hamster hybrid cells. Cell hybrids will be useful in further characterization of NuMA protein.