Analysis of antibody diversity: V‐D‐J mRNA nucleotide sequence of four anti‐GAT monoclonal antibodies. A paucigene system using alternate D‐J recombinations to generate functionally similar hypervariable regions.
Analysis of antibody diversity: V‐D‐J mRNA nucleotide sequence of four anti‐GAT monoclonal antibodies. A paucigene system using alternate D‐J recombinations to generate functionally similar hypervariable regions.
复制标题
抗体多样性分析：四种抗 GAT 单克隆抗体的 V-D-J mRNA 核苷酸序列。使用交替 D-J 重组产生功能相似的高变区的 paucigene 系统。
作者：
J. Rocca;H. Matthes;M. Kaartinen;C. Milstein;J. Thèze;M. Fougereau
测定了4个抗（Glu60‐Ala30‐Tyr10）n （GAT）单克隆γ 1重链mrna从密码子10到120的核苷酸序列。该序列与NH2末端氨基酸序列重叠，从而可以阐明包含VH， D和JH区域的完整蛋白质序列。这些高度保守的序列表明，表达相同公共独特型特异性的抗GAT抗体代表了一个抗原系统，该系统使用至少两个D - J组合，导致参与识别显性Glu - Tyr决定因子的功能相似的高变区。D区由D基因编码，这些基因与D‐SP2或D. fl16种系基因核心密切相关。
The nucleotide sequence of four anti‐(Glu60‐Ala30‐Tyr10)n (GAT) monoclonal gamma 1 heavy chain mRNAs was determined from codon 10 to 120. This sequence overlaps with the NH2‐terminal amino acid sequence, allowing elucidation of the complete protein sequence encompassing regions VH, D and JH. These sequences, which are highly conserved, indicate that anti‐GAT antibodies expressing the same public idiotypic specificities represent a paucigene system, which uses at least two D‐J combinations leading to functionally similar hypervariable regions involved in the recognition of the dominant Glu‐Tyr determinant. D regions are encoded by D genes which are closely related either to the D‐SP2 or the D.FL16 germ line gene cores.