Single-Molecule DNA Analysis
Single-Molecule DNA Analysis
复制标题
DOI：
10.1146/annurev.anchem.111808.073558
复制
发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Thompson, John F.
中科院分区：
文献类型：
--
作者：
Efcavitch, J. William;Thompson, John F.
检测DNA或RNA的单个分子的能力导致了对自然界中最重要的单个生物分子的探索的极其丰富的领域。在核酸分子被拴系到固体支持物、限制在通道中或简单地允许扩散到检测体积中的情况下，已经开发了新的技术来操纵DNA并检查诸如结构动力学和蛋白质-DNA相互作用的性质。除了分析核酸本身的性质外，单分子检测还大大提高了DNA测序的通量，并有望继续取得进展。已经尝试了使用表面、纳米孔和零模式波导的光学和非光学检测方法，并且一种基于光学的仪器已经商业化。与单分子DNA分析相关的文献的广度是巨大的，这篇综述集中在分子动力学和核酸测序的努力调查。
The ability to detect single molecules of DNA or RNA has led to an extremely rich area of exploration of the single most important biomolecule in nature. In cases in which the nucleic acid molecules are tethered to a solid support, confined to a channel, or simply allowed to diffuse into a detection volume, novel techniques have been developed to manipulate the DNA and to examine properties such as structural dynamics and protein-DNA interactions. Beyond the analysis of the properties of nucleic acids themselves, single-molecule detection has enabled dramatic improvements in the throughput of DNA sequencing and holds promise for continuing progress. Both optical and nonoptical detection methods that use surfaces, nanopores, and zero-mode waveguides have been attempted, and one optically based instrument is already commercially available. The breadth of literature related to single-molecule DNA analysis is vast; this review focuses on a survey of efforts in molecular dynamics and nucleic acid sequencing.