Effective inhibition of Salmonella Typhimurium in fresh produce by a phage cocktail targeting multiple host receptors
Effective inhibition of Salmonella Typhimurium in fresh produce by a phage cocktail targeting multiple host receptors
复制标题
DOI：
10.1016/j.fm.2018.08.011
复制
发表时间：
2019-02-01
影响因子：
5.3
通讯作者：
Ryu, Sangryeol
中科院分区：
文献类型：
--
作者：
Bai, Jaewoo;Jeon, Byeonghwa;Ryu, Sangryeol
新鲜农产品的沙门氏菌污染是众多食源性疾病暴发和感染的主要细菌病因。噬菌体可作为天然抗菌剂用于控制食源性病原体。然而，细菌对噬菌体感染的快速耐药性发展是噬菌体实际应用的一个重大障碍。为解决这一问题，我们开发了一种新型噬菌体鸡尾酒，它由三种噬菌体（BSPM4、BSP101和BSP22A）组成，分别针对不同的宿主受体，包括鞭毛、O -抗原和BtuB。对这些噬菌体的全基因组序列分析表明，这三种噬菌体不携带与溶原形成或毒素产生相关的基因，这表明它们作为食品中的生物防治剂是安全的。体外对噬菌体鸡尾酒的处理显著降低了细菌耐药性的产生。在室温（25℃）下12小时后，噬菌体鸡尾酒处理使生菜上的活细胞数减少了4.7 - 5.5 log CFU/cm²，使黄瓜上的活细胞数减少了4.8 - 5.8 log CFU/cm²。该噬菌体鸡尾酒表现出良好的抗菌效果，这表明它可以减少新鲜农产品的鼠伤寒沙门氏菌污染。开发针对多种宿主受体的噬菌体鸡尾酒的策略可用于开发新型鼠伤寒沙门氏菌生物防治剂。
Salmonella contamination of fresh produce is the primary bacterial cause of a significant number of foodborne outbreaks and infections. Bacteriophages can be used as natural antibacterial agents to control foodborne pathogens. However, the rapid development of bacterial resistance to phage infection is a significant barrier to practical phage application. To overcome this problem, we developed a novel phage cocktail consisting of the three phages (BSPM4, BSP101 and BSP22A) that target different host receptors, including flagella, O-antigen and BtuB, respectively. Whole genome sequence analysis of the phages revealed that three phages do not harbor genes involved in lysogen formation or toxin production, suggesting they are safe for use as biocontrol agents in foods. In vitro treatment of the phage cocktail resulted in a significant reduction in the development of bacterial resistance. Phage cocktail treatments achieved 4.7-5.5 log CFU/cm(2) reduction of viable cell number in iceberg lettuce and 4.8-5.8 log CFU/cm(2) reduction in cucumber after 12 h at room temperature (25 degrees C). The phage cocktail exhibited good antimicrobial efficiency, suggesting that it could reduce S. Typhimurium contamination of fresh produce. The strategy of developing cocktails of phages that target multiple host receptors can be used to develop novel biocontrol agents of S. Typhimurium.