Stopped-flow investigation of antioxidant activity of tocopherols.
Stopped-flow investigation of antioxidant activity of tocopherols.
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生育酚抗氧化活性的停流研究。
DOI：
10.1246/bcsj.59.3113
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发表时间：
1986
影响因子：
4
通讯作者：
K. Ishizu
中科院分区：
文献类型：
--
作者：
K. Mukai;Yoshiko Watanabe;Y. Uemoto;K. Ishizu
用ESR测量观察到，α-、β-、γ-和δ-生育酚（维生素E）在苯中被稳定的苯氧基自由基氧化，立即生成相应的生育酚氧基自由基。用停流技术测定了α-、β-、γ-和δ-生育酚在乙醇溶液中与稳定的苯氧基自由基的反应速率，作为生育酚与生物体系中不稳定自由基（ROO·、RO·和HO·）的模型反应。在25.0 °C乙醇溶液中测得的二级速率常数分别为（5.12±0.36）×103（α-Toc），（2.24±0.04）×103（β-Toc），（2.42±0.16）×103（γ-Toc）和（0.51±0.01）×103（δ-Toc），M−1 s−1。的相对速率同意与生育酚对聚（苯乙烯基过氧）和galvinoxyl自由基的反应性的研究所获得的O2消耗和ESR方法，分别。结果表明，生育酚的相对反应活性，即相对抗氧化活性与不稳定自由基的种类无关。
It was observed by ESR measurement that the oxidation of α-, β-, γ-, and δ-tocopherols (vitamin E) with a stable phenoxyl radical in benzene immediately gives corresponding tocopheroxyl radicals. The rates of reaction of α-, β-, γ-, and δ-tocopherols with the stable phenoxyl radical in ethanol solution have been determined spectrophotometrically using stopped-flow technique, as a model reaction of tocopherols with unstable free radicals (ROO·, RO·, and HO·) in biological systems. The second-order rate constants obtained are (5.12±0.36)×103 (α-Toc), (2.24±0.04)×103 (β-Toc), (2.42±0.16)×103 (γ-Toc), and (0.51±0.01)×103 (δ-Toc), M−1 s−1 in ethanol at 25.0 °C. The relative rates agree well with those obtained from studies of the reactivities of tocopherols toward poly(styrylperoxyl) and galvinoxyl radicals by O2 consumption and by ESR method, respectively. The results suggest that the relative reactivities, that is, relative antioxidant activities of tocopherols do not depend on the kinds of unstable free rad...
α-生育酚及其模型化合物与超氧离子反应自由基的电子自旋共振研究
DOI：
10.1016/0005-2760(78)90183-2
发表时间：
1978
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
Ozawa Toshihiko;Hanaki Akira;Matsumoto Shigenobu;Matsuo Mitsuyoshi
通讯作者：
Matsuo Mitsuyoshi