Degradation of glomerular basement membrane by human neutrophils in vitro.
Degradation of glomerular basement membrane by human neutrophils in vitro.
复制标题
人中性粒细胞体外降解肾小球基底膜。
DOI：
10.1016/0167-4889(84)90144-7
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
J. S. Hunt
中科院分区：
文献类型：
--
作者：
M. Vissers;C. Winterbourn;J. S. Hunt
The glomerular basement membrane is susceptible to immunologic injury when immune complexes or anti-basement-membrane antibodies become lodged in its network. We have studied the digestion of glomerular basement membrane prepared from normal human kidney by isolated neutrophils. In the absence of immunoglobulin aggregates or immune complexes, there was little evidence of neutrophil adherence to the membrane, of release of lysosomal enzymes, or of digestion. However, when the basement membrane contained immunoglobin G (IgG) aggregates generated in situ by heating the membrane impregnated with IgG to 63°C, electron micrographs showed neutrophils adherent to the basement-membrane surface and phagocytosis of smaller fragments. Lysosomal enzymes were detectable in the extracellular medium, and measurements of either total protein or hydroxyproline solubilized showed digestion of 80 μg basement membrane/h per 107cells. Hydroxyproline solubilization was almost totally inhibited by phenylmethylsulphonyl fluoride, indicating that the neutrophil serine proteinases, elastase and cathepsin G are responsible for degradation. These findings provide direct evidence for the digestion of extracellular matrix by neutrophils stimulated in situ by deposited immune complexes as a contributor to inflammatory tissue damage.
