Electrical lysis of cells for detergent-free droplet assays.
Electrical lysis of cells for detergent-free droplet assays.
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DOI：
10.1063/1.4944742
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发表时间：
2016-03
期刊：
影响因子：
3.2
通讯作者：
Abate AR
中科院分区：
文献类型：
--
作者：
de Lange N;Tran TM;Abate AR
当分析微流体液滴中的单细胞时，有效的裂解是至关重要的，但是现有的方法利用可能干扰待进行的测定的去污剂。我们证明了在不使用洗涤剂或其他化学品的情况下稳健的细胞裂解。在我们的方法中，细胞在封装在液滴中之前立即暴露于电场，导致细胞裂解。我们将裂解效率表征为控制参数的函数，并通过测量β-葡萄糖苷酶（一种用于将生物质转化为生物燃料的重要纤维素酶）的催化来证明与酶测定的相容性。我们的方法能够在与洗涤剂不相容的微流体液滴中进行测定。
Efficient lysis is critical when analyzing single cells in microfluidic droplets, but existing methods utilize detergents that can interfere with the assays to be performed. We demonstrate robust cell lysis without the use of detergents or other chemicals. In our method, cells are exposed to electric field immediately before encapsulation in droplets, resulting in cell lysis. We characterize lysis efficiency as a function of control parameters and demonstrate compatibility with enzymatic assays by measuring the catalysis of β-glucosidase, an important cellulase used in the conversion of biomass to biofuel. Our method enables assays in microfluidic droplets that are incompatible with detergents.