Increased nitrotyrosine in exhaled breath condensate of patients with asthma
Increased nitrotyrosine in exhaled breath condensate of patients with asthma
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DOI：
10.1164/ajrccm.162.4.9912064
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发表时间：
2000-10-01
影响因子：
24.7
通讯作者：
Barnes, PJ
中科院分区：
文献类型：
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作者：
Hanazawa, T;Kharitonov, SA;Barnes, PJ
一氧化氮（NO）和超氧阴离子（O-2（-））在气道中的反应导致过氧亚硝酸盐的形成，这是一种高度反应性的氧化剂。过氧亚硝酸盐与蛋白质中的酪氨酸残基反应形成稳定的产物硝基酪氨酸。我们研究了哮喘患者呼出气冷凝物中硝基酪氨酸是否会增加。研究了四组不吸烟的受试者。我们测量了健康的非特应性受试者（n = 15）和轻度哮喘（类固醇初治，n = 15）、中度哮喘（吸入类固醇治疗，n = 12）和重度哮喘（口服类固醇治疗，n = 12）患者呼气冷凝液中的NO、硝基酪氨酸和白三烯浓度。轻、中度哮喘患者呼出气中NO含量分别为19.2 ± 2.7ppb和14.0 ± 1.53ppb，与正常对照组（6.58 ± 0.61ppb）比较，差异有显著性（P < 0.01和P < 0.05）。中、重度哮喘患者经激素治疗后，LTC 4/D-4/E-4和LTB 4水平明显升高。在正常人的呼气冷凝液中可检测到硝基酪氨酸浓度（6.3 ± 0.8 ng/ml），轻度哮喘患者的浓度显著升高（15.3 ± 2.0 ng/ml，p < 0.01）。然而，呼出冷凝液中的硝基酪氨酸水平在中度（5.0 ± 0.6 ng/ml）和重度哮喘患者（3.3 ± 0.6 ng/ml，p < 0.05）中较低。轻度哮喘患者呼气冷凝液中硝基酪氨酸与呼出气中NO含量呈显著相关（r = 0.65，p < 0.05）。我们的结论是，呼出气冷凝物中硝基酪氨酸的形成可能是哮喘气道氧化应激的一个标志。
The reaction of nitric oxide (NO) and superoxide anions (O-2(-)) in the airway results in the formation of peroxynitrite, a highly reactive oxidant species. Peroxynitrite reacts with tyrosine residues in proteins to form the stable product nitrotyrosine. We investigated whether nitrotyrosine in exhaled breath condensates may be increased in patients with asthma. four groups of nonsmoking subjects were studied. We measured exhaled NO, nitrotyrosine, and leukotrienes concentrations in breath condensate in healthy nonatopic subjects (n = 15) and in patients with mild asthma (steroid naive, n = 15), moderate asthma (inhaled steroid treatment, n = 12), and severe asthma (oral steroid treatment, n = 12). Exhaled NO was increased significantly In patients with mild (19.2 +/- 2.7 ppb, p < 0.01) and moderate asthma (14.0 +/- 1.53 ppb, p < 0.05), as compared with normal control (6.58 +/- 0.61 ppb). The levels of LTC4/D-4/E-4 and LTB4 were increased significantly in patients with moderate and severe asthma treated with steroids. Nitrotyrosine concentrations were detectable (6.3 +/- 0.8 ng/ml) in breath condensate of normal subjects, and were increased significantly in patients with mild asthma (15.3 +/- 2.0 ng/ml, p < 0.01). However, the levels of nitrotyrosine in exhaled condensate were lower in patients with moderate (5.0 +/- 0.6 ng/ml) and severe asthma (3.3 +/- 0.6 ng/ml, p < 0.05). There was a significant correlation between nitrotyrosine in breath condensate and exhaled NO in patients with mild asthma (r = 0.65, p < 0.05). We conclude that nitrotyrosine formation in exhaled breath condensates may be a marker of oxidative stress in airways of asthma.