Changes in phospholipase D expression in soybeans during seed development and germination
Changes in phospholipase D expression in soybeans during seed development and germination
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DOI：
10.1007/bf02523380
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发表时间：
1996-09-01
影响因子：
2
通讯作者：
Wang, XM
中科院分区：
文献类型：
--
作者：
Ryu, SB;Zheng, L;Wang, XM
磷脂酶D （PLD）的激活与非水合磷脂的积累和脂质降解有关，导致大豆种子在收获前和收获后变质。本研究检测了大豆种子发育和萌发过程中PLD活性、蛋白和mRNA的变化。RNA印迹分析表明，编码PLD的基因在种子发育的早期和中期表达量最高。然而，每子叶积累的PLD活性在成熟种子中达到最高水平。在萌发和幼苗早期生长过程中，吸胀后1天未检测到PLD mRNA，而3天和7天幼苗的子叶中PLD表达量显著增加。同样，吸胀后第1天PLD活性和蛋白浓度变化不大，吸胀后3天和7天幼苗PLD活性和蛋白浓度升高。这些结果表明，PLD的表达受发育调控，其活性和蛋白量的变化主要受mRNA水平的控制，免疫印迹分析进一步揭示了PLD变体的存在，这些变体与种子发育和幼苗生长的特定阶段有关。成熟种子子叶中存在的PID变异似乎与种子发育早期和幼苗中观察到的不同。
Activation of phospholipase D (PLD) has been linked to accumulation of nonhydratable phosphatides and lipid degradation leading to soybean seed deterioration during preharvest and postharvest events. This study examined the changes in PLD activity, protein, and mRNA in soybeans during seed development and germination. RNA blotting analysis indicated that expression of the gene that encodes PLD was highest during the early and middle stages of seed development. However, the amount of PLD activity accumulated per cotyledon reached the highest level in mature seeds. During germination and early seedling growth, PLD mRNA was not detected one day after imbibition, while a significant increase in PLD expression occurred in the cotyledons of three- and seven-day seedlings, Similarly, PLD activity and protein concentration showed little change during the first day of imbibition and increased afterward in three- and seven-day seedlings. These results suggested that expression of PLD is developmentally regulated and that the changes in its amount of activity and protein are controlled primarily at the mRNA level, Immunoblotting analysis further revealed the presence of PLD variants that were associated with specific stages of seed development and seedling growth. The PID variants present in the cotyledons of mature seeds appeared to be distinct from those observed in the early stage of seed development and in young seedlings.