Autoantibodies Against Stress-Induced Phosphoprotein-I as a Novel Biomarker Candidate for Ovarian Cancer
Autoantibodies Against Stress-Induced Phosphoprotein-I as a Novel Biomarker Candidate for Ovarian Cancer
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DOI：
10.1002/gcc.20769
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发表时间：
2010-07-01
影响因子：
3.7
通讯作者：
Kim, Jae-Hoon
中科院分区：
文献类型：
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作者：
Kim, Sunghoon;Cho, HanByoul;Kim, Jae-Hoon
肿瘤相关抗原（TAA）自身抗体的检测最近已被证明是一个强大的工具，用于各种癌症的早期检测。本研究的目的是通过基于蛋白质组学的方法，研究在卵巢癌患者中使用抗TAA自身抗体作为新型生物标志物的可能性。我们采用免疫沉淀肿瘤抗原（2D-DITA）的二维差异凝胶电泳分析，比较卵巢癌患者治疗前和治疗后血清中自体抗体的水平。通过SYBR绿色实时聚合酶链反应（PCR）和免疫组织化学（IHC）验证鉴定的自身抗体。我们进一步评估了68例卵巢癌患者血清中的自身抗体水平的酶联免疫吸附试验（ELISA）。针对应激诱导的磷蛋白-I（STIP-1）的自身抗体成为卵巢癌的新的生物标志物候选者。SYBR绿色PCR和免疫组化证实STIP-1 mRNA和蛋白在卵巢癌组织中的表达水平明显高于正常卵巢组织和良性卵巢肿瘤组织（P = 0.003和P < 0.001）。初步的ELISA研究显示，卵巢癌患者血清中抗STIP-1自身抗体水平显著高于健康对照组（P = 0.03）。结果表明，2D-DITA是检测自身抗体的有用工具，STIP-1是卵巢癌的潜在生物标志物候选者。(C)2010 Wiley-Liss，Inc.
Detection of autoantibodies against tumor-associated antigens (TAA) has recently been shown to be a powerful tool for early detection of various cancers. The aim of this study was to investigate the possibility of using autoantibodies against TAA as novel biomarkers by a proteomics-based approach in patients with ovarian cancer. We used two-dimensional differential gel electrophoresis analysis of immuno-precipitated tumor antigens (2D-DITA) to compare the levels of autoandibodies in pretreatment and posttreatment sera of patients with ovarian cancers. The identified autoantibodies were validated by SYBR Green real-time polymerase chain reaction (PCR) and immunohistochemistry (IHC). We further evaluated the level of autoantibody in sera of 68 ovarian cancer patients by an enzyme-linked immunosorbent assay (ELISA). The autoantibody directed against stress-induced phosphoprotein-I (STIP-1) emerged as a novel biomarker candidate for ovarian cancer. SYBR Green PCR and IHC confirmed that the STIP-1 mRNA and protein expression levels were significantly up-regulated in ovarian cancers compared with normal and benign tumors (P = 0.003 and P < 0.001, respectively). A preliminary ELISA study showed that the serum levels of anti-STIP-1 autoantibodies were significantly elevated in ovarian cancer patients compared with healthy controls (P = 0.03). The results suggest that 2D-DITA is a useful tool to detect autoantibodies and that STIP-1 is a potential biomarker candidate for ovarian cancers. (C) 2010 Wiley-Liss, Inc.