EXPERIMENTAL RABIES - STUDIES OF CELLULAR VULNERABILITY AND PATHOGENESIS USING FLUORESCENT ANTIBODY STAINING
EXPERIMENTAL RABIES - STUDIES OF CELLULAR VULNERABILITY AND PATHOGENESIS USING FLUORESCENT ANTIBODY STAINING
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DOI：
10.1097/00005072-196510000-00009
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发表时间：
1965-01-01
影响因子：
3.2
通讯作者：
JOHSNON, RT
中科院分区：
文献类型：
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作者：
JOHSNON, RT
在常规组织学和病毒学方法的基础上，采用荧光抗体染色法对小鼠固定狂犬病病毒感染进行了研究。脑脊液/天花病毒仅在神经元中检测到，感染主要局限于脑脊液结构、脑干核、背根神经节、前角和小脑浦肯野细胞的神经元。在潜伏期内，抗原在敏感神经元的核周和树突内缓慢形成，在轴突内未发现抗原。尽管在神经元内有大量的病毒发展，但神经元吞噬很少，没有炎症反应。接种s/c后，发现病毒沿周围神经迅速扩散到中枢神经系统，而没有证据表明感染神经内膜细胞或任何神经外组织。这些发现与其他实验病毒性脑碱观察到的结果不同，并讨论了这些独特性质的可能解释。(作为)。
Fixed rabies virus infections of mice were studied using fluorescent antibody staining in addi tion to conventional histological and virological methods. Following intracerebral ors/cinoculation virus was detectable only in neurones, and infection was limited primarily to neurones of rhinencephalic structures, brain stem nuclei, dorsal root ganglia, anterior horns, and cerebellar Purkinje cells. During the incubation period, antigen developed slowly ovei five days within the perikaryon and dendrites of susceptible neurones, and no antigen was found in axones. Despite the development of large amounts of virus within neurones, there was a paucity of neuronophagia and no inflammatory reaction. Fol lowing s/c inoculation virus was found to spread rapidly to the c.n.s. along peripheral nerves without evidence of infection of the endoneural cells or any extraneural tissues. These findings are different from those observed with other experimental viral encephalitides, and possible explanations of these unique properties are discussed. (AS).