DISTRIBUTION OF INTRAVENTRICULARLY INJECTED HORSERADISH-PEROXIDASE IN CEREBROSPINAL-FLUID COMPARTMENTS OF THE RAT SPINAL-CORD
DISTRIBUTION OF INTRAVENTRICULARLY INJECTED HORSERADISH-PEROXIDASE IN CEREBROSPINAL-FLUID COMPARTMENTS OF THE RAT SPINAL-CORD
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DOI：
10.1007/bf00645050
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发表时间：
1992-12-01
影响因子：
3.6
通讯作者：
RODRIGUEZ, EM
中科院分区：
文献类型：
--
作者：
CIFUENTES, M;FERNANDEZLLEBREZ, P;RODRIGUEZ, EM
用辣根过氧化物酶（HRP）注入大鼠侧脑室，观察了脑脊液沿着中央管的循环及其进入脊髓实质的途径。注射后13 min，在整个中央管中发现过氧化物酶，表明脑脊液沿着大鼠脊髓中央管快速循环。它在2小时内从中央管中清除，与脑组织中的情况相反，它在脑室周围区域保留了4小时。在中央管，HRP结合到Reissner纤维和室管膜细胞的腔表面，它穿过室管膜衬里的细胞间隙，到达室管膜下神经鞘，局部毛细血管的基底膜，并出现在内皮细胞胞饮小泡的腔中。在室管膜细胞的基底外侧面与基底膜接触处也有大量蓄积。在室管膜细胞，HRP被发现在单个吞饮囊泡。供应脊髓的血管分为两种类型。A型血管从侧面和背侧穿过脊髓，并沿其外壁沿着显示示踪剂，直至灰质。B型血管从内侧腹侧沟进入脊髓，占据灰质前连合，接近中央管。它们是唯一一条在灰质中沿着被HRP标记的血管。HRP从B型血管壁开始扩散，标记了室管膜内层的细胞间隙和中央管腔。这表明中央管和外部脑脊液空间之间的沟通，在内侧腹侧沟的水平，通过细胞间隙，血管周围基底膜及其血管延伸。
The circulation of the cerebrospinal fluid along the central canal and its access to the parenchyma of the spinal cord of the rat have been analyzed by injection of horseradish peroxidase (HRP) into the lateral ventricle. Peroxidase was found throughout the central canal 13 min after injection, suggesting a rapid circulation of cerebrospinal fluid along the central canal of the rat spinal cord. It was cleared from the central canal within 2 h, in contrast with the situation in the brain tissue, where it remained in the periventricular areas for 4 h. In the central canal, HRP bound to Reissner's fiber and the luminal surface of the ependymal cells; it penetrated through the intercellular space of the ependymal lining, reached the subependymal neuropil, the basement membrane of local capillaries, and appeared in the lumen of endothelial pinocytotic vesicles. Furthermore, it accumulated in the labyrinths of the basement membrane contacting the basolateral aspect of the ependymal cells. In ependymocytes, HRP was found in single pinocytotic vesicles. The blood vessels supplying the spinal cord were classified into two types. Type-A vessels penetrated the spinal cord laterally and dorsally and displayed the tracer along their external wall as far as the gray matter. Type-B vessels intruded into the spinal cord from the medial ventral sulcus and occupied the anterior commissure of the gray matter, approaching the central canal. They represented the only vessels marked by HRP along their course through the gray matter. HRP spread from the wall of type-B vessels, labeling the labyrinths, the intercellular space of the ependymal lining, and the lumen of the central canal. This suggests a communication between the central canal and the outer cerebrospinal fluid space, at the level of the medial ventral sulcus, via the intercellular spaces, the perivascular basement membrane and its labyrinthine extensions.