Expression of CD200 on epithelial cells of the murine hair follicle: A role in tissue-specific immune tolerance?
Expression of CD200 on epithelial cells of the murine hair follicle: A role in tissue-specific immune tolerance?
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DOI：
10.1111/j.0022-202x.2004.23461.x
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发表时间：
2004-11-01
影响因子：
6.5
通讯作者：
Truitt, RL
中科院分区：
文献类型：
--
作者：
Rosenblum, MD;Olasz, EB;Truitt, RL
CD 200（OX-2）是一种跨膜糖蛋白，通过CD 200受体（CD 200 R）传递免疫调节信号，以减轻炎症反应并促进免疫耐受。皮肤中的CD 200表达以前没有描述过。我们现在报告说，新鲜分离的小鼠表皮细胞含有一个亚群的主要组织相容性复合体（MHC）II类阴性，CD 3阴性的角质形成细胞是CD 200阳性。CD 200的表达在包括鼠毛囊（HF）的外根鞘的角质形成细胞中加重。当在性别匹配的野生型（WT）和CD 200缺陷型C57 BL/6小鼠之间交换同基因皮肤移植物时，观察到显著的毛囊周围和毛囊内炎症，最终导致几乎所有HF（脱发）的破坏，而CD 200阴性移植物没有显著损失。在WT移植物中观察到极轻微和短暂的炎症，其在毛发中长期存在。在14 d时，CD 200缺陷皮肤中的移植物浸润T细胞增加了2倍。在CD 200缺陷型宿主中，通过过继转移来自先前移植有CD 200阴性皮肤的WT小鼠的脾细胞诱导脱发和皮肤病变，但不从移植有WT皮肤的小鼠中诱导。总的来说，这些结果表明，CD 200在滤泡上皮细胞的表达减弱炎症反应，并可能在维持免疫耐受HF相关的自身抗原的作用。
CD200 (OX-2) is a transmembrane glycoprotein that transmits an immunoregulatory signal through the CD200 receptor (CD200R) to attenuate inflammatory reactions and promote immune tolerance. CD200 expression in the skin has not been described previously. We now report that freshly isolated cells of the murine epidermis contain a subpopulation of major histocompatibility complex (MHC) class II-negative, CD3-negative keratinocytes that are CD200-positive. CD200 expression was accentuated in keratinocytes comprising the outer root sheath of the murine hair follicle (HF). When syngeneic skin grafts were exchanged between gender-matched wild-type (WT) and CD200-deficient C57BL/6 mice, significant perifollicular and intrafollicular inflammation was observed, eventually leading to the destruction of virtually all HF (alopecia) without significant loss of the CD200-negative grafts. Minimal and transient inflammation was observed in WT grafts, which persisted long term with hair. There was a 2-fold increase in graft-infiltrating T cells in CD200-deficient skin at 14 d. Alopecia and skin lesions were induced in CD200-deficient hosts by adoptive transfer of splenocytes from WT mice previously grafted with CD200-negative skin, but not from mice grafted with WT skin. Collectively, these results suggest that the expression of CD200 in follicular epithelium attenuates inflammatory reactions and may play a role in maintaining immune tolerance to HF-associated autoantigens.