Gold-DNA nanosunflowers for efficient gene silencing with controllable transformation
Gold-DNA nanosunflowers for efficient gene silencing with controllable transformation
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金-DNA 纳米向日葵通过可控转化实现高效基因沉默
DOI：
10.1126/sciadv.aaw6264
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发表时间：
2019-10-01
期刊：
影响因子：
13.6
通讯作者：
Liang, Xing-Jie
中科院分区：
文献类型：
--
作者：
Huo, Shuaidong;Gong, Ningqiang;Liang, Xing-Jie
开发用于增强和受控基因干扰疗法的有效递送系统仍然面临着巨大的挑战。幸运的是，蓬勃发展的纳米技术领域为核酸递送提供了更有效的策略。在这里，形成三链体的寡核苷酸序列及其互补链用于介导超小型金纳米颗粒的自组装。获得的向日葵状纳米结构表现出很强的近红外（NIR）吸收和光热转换能力。在近红外辐射下，大尺寸纳米结构可以分解并生成用c-myc癌基因沉默序列修饰的超小纳米颗粒，可以直接靶向细胞核。此外，通过协同控制自组装纳米结构的预孵育时间（体外和体内）和近红外照射时间点，可以实现受控的基因沉默效果。这项研究为构建用于基因干扰应用的更有效和可定制的纳米载体提供了一种新方法。
The development of an efficient delivery system for enhanced and controlled gene interference-based therapeutics is still facing great challenges. Fortunately, the flourishing field of nanotechnology provides more effective strategies for nucleic acid delivery. Here, the triplex-forming oligonucleotide sequence and its complementary strand were used to mediate self-assembly of ultrasmall gold nanoparticles. The obtained sunflower-like nanostructures exhibited strong near-infrared (NIR) absorption and photothermal conversion ability. Upon NIR irradiation, the large-sized nanostructure could disassemble and generate ultrasmall nanoparticles modified with c-myc oncogene silencing sequence, which could directly target the cell nucleus. Moreover, the controlled gene silencing effect could be realized by synergistically controlling the preincubation time with the self-assembled nanostructure (in vitro and in vivo) and NIR irradiation time point. This study provides a new approach for constructing more efficient and tailorable nanocarriers for gene interference applications.