Benzo[a]pyrene exposure increases toxic biomarkers and morphological disorders in mouse cervix.
Benzo[a]pyrene exposure increases toxic biomarkers and morphological disorders in mouse cervix.
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DOI：
10.1111/j.1742-7843.2011.00755.x
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发表时间：
2011-11
影响因子：
3.1
通讯作者：
Meili Gao;Yongfei Li;Ying Sun;Walayat Shah;Shuiyun Yang;Yili Wang;J. Long
中科院分区：
文献类型：
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作者：
Meili Gao;Yongfei Li;Ying Sun;Walayat Shah;Shuiyun Yang;Yili Wang;J. Long
苯并[a]芘（BaP）是多环芳烃的代表性化合物，对人类肝脏、肺、胃和皮肤具有细胞毒性和遗传毒性。然而，BaP 对宫颈组织的毒性作用仍不清楚。本研究旨在研究 BaP 对 ICR 小鼠子宫颈的毒性作用。雌性小鼠以2.5、5和10 mg/kg体重的剂量腹腔注射和口服BaP治疗，每周两次，持续14周。 BaP 治疗导致子宫颈和肝脏中 MDA 和 IL-6 水平显着升高，CYP1A1、肌酸激酶和天冬氨酸转氨酶 (AST) 活性显着升高，谷胱甘肽-S-转移酶活性降低。腹腔注射 BaP 组的相对宫颈重量显着降低，而口服灌胃组仅观察到轻微降低。体重增加随着BaP剂量的增加而减少。此外，BaP治疗还会引起宫颈组织显着的病理形态学变化，并增加小鼠的死亡率。综上所述，这些结果表明 BaP 对宫颈组织造成一定的毒性作用。
Benzo[a]pyrene (BaP) is a representative compound of polycyclic aromatic hydrocarbons exerting cytotoxicity and genotoxicity in the human liver, lung, stomach and skin. However, the toxic effect of BaP on cervical tissue remains unclear. This study was carried out to investigate the toxic effects of BaP on the cervix of ICR mice. Female mice were treated with BaP by intraperitoneal injection and oral gavage at a dose of 2.5, 5 and 10 mg/kg body-weight, twice a week for 14 weeks. BaP treatment caused a significant increase in the levels of MDA and IL-6 with significantly increased activity of CYP1A1, creatine kinase and aspartate aminotransferase (AST) and decreased activity of glutathione-S-transferase in the cervix and liver. The relative cervix weight was markedly reduced in the intraperitoneal BaP injection groups, whereas only a slight reduction was observed in the oral gavage groups. The increase in weight decreased with increasing BaP dose. Moreover, BaP treatment induced significant pathomorphological changes in the cervical tissue and increased the mortality of the mice. Taken together, these results suggest that BaP causes a certain toxic effect on cervical tissue.