Differentiation of myeloid dendritic cells into CD8alpha-positive dendritic cells in vivo.
Differentiation of myeloid dendritic cells into CD8alpha-positive dendritic cells in vivo.
复制标题
DOI：
10.1182/blood.v96.5.1865
复制
发表时间：
2000
期刊：
影响因子：
20.3
通讯作者：
M. Merad;L. Fong;J. Bogenberger;E. Engleman
中科院分区：
文献类型：
--
作者：
M. Merad;L. Fong;J. Bogenberger;E. Engleman
骨髓来源的树突状细胞 (DC) 代表具有不同表型的抗原呈递细胞 (APC) 家族。例如，在小鼠中，CD8α(+) 和 CD8α(-) DC 被认为分别代表淋巴和骨髓来源的细胞。表皮朗格汉斯细胞 (LC) 是典型的髓样 DC；它们不表达 CD8α，但表达高水平的骨髓抗原，例如 CD11b 和 FcgammaR。相比之下，源自淋巴相关祖细胞的胸腺 DC 表达 CD8α，但仅表达低水平的骨髓抗原。 CD8α(+) DC 也存在于脾脏和淋巴结 (LN) 中，但这些细胞的来源尚未确定。通过体内激活和标记CD8α(-)表皮LC，发现这些细胞在迁移至引流淋巴结时表达CD8α。类似地，CD8α(-) LC由CD8野生型小鼠体外产生，并注射到CD8αKO小鼠的皮肤中，当它们到达引流淋巴结时表达CD8α。结果还表明 CD8alpha(+) LC 是有效的 APC。从皮肤迁移后，它们定位于 LN 的 T 细胞区域，分泌高水平的白介素 12、干扰素 γ 和趋化因子吸引 T 细胞，并诱导抗原特异性 T 细胞激活。这些结果表明，外周的骨髓 DC 在迁移到引流淋巴结时可以表达 CD8α。这些 DC 上的 CD8α 表达似乎反映了激活、动员或两者的状态，而不是谱系。 （血。2000；96：1865-1872）
Bone marrow-derived dendritic cells (DC) represent a family of antigen-presenting cells (APC) with varying phenotypes. For example, in mice, CD8alpha(+) and CD8alpha(-) DC are thought to represent cells of lymphoid and myeloid origin, respectively. Langerhans cells (LC) of the epidermis are typical myeloid DC; they do not express CD8alpha, but they do express high levels of myeloid antigens such as CD11b and FcgammaR. By contrast, thymic DC, which derive from a lymphoid-related progenitor, express CD8alpha but only low levels of myeloid antigens. CD8alpha(+) DC are also found in the spleen and lymph nodes (LN), but the origin of these cells has not been determined. By activating and labeling CD8alpha(-) epidermal LC in vivo, it was found that these cells expressed CD8alpha on migration to the draining LN. Similarly, CD8alpha(-) LC generated in vitro from a CD8 wild-type mouse and injected into the skin of a CD8alphaKO mouse expressed CD8alpha when they reached the draining LN. The results also show that CD8alpha(+) LC are potent APC. After migration from skin, they localized in the T-cell areas of LN, secreted high levels of interleukin-12, interferon-gamma, and chemokine-attracting T cells, and they induced antigen-specific T-cell activation. These results demonstrate that myeloid DC in the periphery can express CD8alpha when they migrate to the draining LN. CD8alpha expression on these DC appears to reflect a state of activation, mobilization, or both, rather than lineage. (Blood. 2000;96:1865-1872)