Sex difference in norepinephrine surge in response to psychological stress through nitric oxide in rats
Sex difference in norepinephrine surge in response to psychological stress through nitric oxide in rats
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DOI：
10.1016/j.lfs.2006.11.011
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发表时间：
2007-02-06
期刊：
影响因子：
6.1
通讯作者：
Morimoto, Keiko
中科院分区：
文献类型：
--
作者：
Uji, Masami;Yoshida, Ken-ichi;Morimoto, Keiko
心理压力通过激活交感神经使血压升高。这种升压反应被认为与心血管事件有关。我们研究了大鼠对笼子转换应激的升压反应和去甲肾上腺素激增的性别差异。对Wistar雄性和雌性大鼠插管进行血压监测和血液采样。手术后6天，将大鼠暴露于换笼应激，并在休息时和应激开始后30分钟收集血液样品。雄性大鼠的应激性升压反应大于雌性大鼠。应激显著增加雄性大鼠的去甲肾上腺素水平，但雌性大鼠没有。用一氧化氮（NO）合酶抑制剂N-硝基-L-精氨酸甲酯（L-NAME）预处理雄性大鼠，可显著减弱去甲肾上腺素反应。腓肠肌内皮型一氧化氮合酶表达无性别差异。然而，丝氨酸1177的磷酸化，eNOS激活的标志物，在雄性大鼠中高于雌性大鼠。提示NO参与了雄性大鼠心理应激时去甲肾上腺素激增的过程，而雌性大鼠无此作用。这是第一个报告的性别差异，去甲肾上腺素激增的心理压力，通过NO，与升压反应。(c)2006年爱思唯尔公司All rights reserved.
Psychological stress elevates blood pressure through sympathetic nerve activation. This pressor response is supposedly associated with cardiovascular events. We investigated a sex difference in the pressor response and norepinephrine surge to cage-switch stress in rats. Wistar male and female rats were catheterized for blood pressure monitoring and blood sampling. Six days post-surgery, the rats were exposed to the cage-switch stress and blood samples were collected at rest and 30 min after the start of the stress. The stress-induced pressor response was greater in the male than in the female rats. The stress significantly increased the norepinephrine level in the male, but not in the female rats. Pre-treatment with N-G-nitro-L-arginine methyl ester (L-NAME), a nitric oxide (NO) synthase inhibitor, attenuated the norepinephrine response significantly in the male rats. There was no sex difference in the endothelial NO synthase expression in the gastrocnemius muscle. However the phosphorylation at serine 1177, a marker for eNOS activation, was higher in the male than in the female rats. These results suggest that NO is involved in the norepinephrine surge to psychological stress in the male rats, but not in the female rats. This is the first report on a sex difference in the norepinephrine surge in response to psychological stress through NO, in association with pressor response. (c) 2006 Elsevier Inc. All rights reserved.