Determination of deoxymyoglobin changes during graded myocardial ischemia: An in vivo H-1 NMR spectroscopy study
Determination of deoxymyoglobin changes during graded myocardial ischemia: An in vivo H-1 NMR spectroscopy study
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DOI：
10.1002/mrm.1910380206
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发表时间：
1997-08-01
影响因子：
3.3
通讯作者：
Ugurbil, K
中科院分区：
文献类型：
--
作者：
Chen, W;Zhang, JY;Ugurbil, K
应用1H-1 NMR技术检测了犬在体心肌分级缺血时脱氧肌红蛋白近端组氨酰N-δ质子信号，其信号强度可作为心肌细胞内氧合的指标。在静息、部分和完全冠状动脉闭塞条件下，成功地测量了心肌血流量与脱氧肌红蛋白浓度之间的关系。结果表明，首次在活体心脏中使用1H NMR光谱技术检测脱氧肌红蛋白是可行的，并且可能使用这种技术来非破坏性地和重复地研究心肌氧化代谢。
H-1 NMR spectroscopy was used to detect the proximal histidyl N-delta proton signal of deoxymyoglobin from canine hearts in vivo during graded myocardial ischemia, The NMR signal intensity provided an indicator of intracellular oxygenation in myocardium. The relationship between the myocardial blood flow and the deoxymyoglobin concentration was successfully measured during resting, partial, and complete coronary artery occlusion conditions, The results demonstrate the feasibility to detect deoxymyoglobin using a 1H NMR spectroscopy technique in living hearts for the first time and the possibility to use this technique for investigating myocardial oxidative metabolism nondestructively and repetitively.