An evolutionary intra-molecular shift in the preferred U3 snoRNA binding site on pre-ribosomal RNA.
An evolutionary intra-molecular shift in the preferred U3 snoRNA binding site on pre-ribosomal RNA.
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DOI：
10.1093/nar/gki815
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发表时间：
2005
影响因子：
14.9
通讯作者：
Gerbi SA
中科院分区：
文献类型：
--
作者：
Borovjagin AV;Gerbi SA
U3小核核RNA （snoRNA）在核糖体前RNA （pre-rRNA）上的正确对接对于rRNA加工产生18S rRNA至关重要。在本报告中，我们使用非洲爪蟾卵母细胞来表征U3 snoRNA 3 ' -hinge与pre-rRNA外部转录间隔区（ETS） E1区相互作用的结构要求。这种相互作用对于对接启动rRNA加工至关重要。当与ETS形成的u33 ' -铰链复合物的8 bp中少于6个时，18S rRNA的产生受到抑制；此外，涉及3 ' -铰链右侧的碱基配对比涉及左侧的碱基配对更重要。将U3 - ets相互作用的长度增加9 bp会损害rRNA的加工。通过交换与ETS的U3 5′-和3′-铰链相互作用，或通过将U3 3′-铰链的碱基对转移到与ETS区域E1直接相邻的序列，也可以抑制18S rRNA的形成。然而，18S rRNA的产生通过补偿性移位得以部分恢复，该移位允许靠近U3 3 ' -hinge的序列与直接靠近ETS区域E1的8个碱基配对。结果表明，U3 snoRNA与ETS相互作用的几何结构对rRNA加工至关重要。
Correct docking of U3 small nucleolar RNA (snoRNA) on pre-ribosomal RNA (pre-rRNA) is essential for rRNA processing to produce 18S rRNA. In this report, we have used Xenopus oocytes to characterize the structural requirements of the U3 snoRNA 3′-hinge interaction with region E1 of the external transcribed spacer (ETS) of pre-rRNA. This interaction is crucial for docking to initiate rRNA processing. 18S rRNA production was inhibited when fewer than 6 of the 8 bp of the U3 3′–hinge complex with the ETS could form; moreover, base pairing involving the right side of the 3′-hinge was more important than the left. Increasing the length of the U3 hinge–ETS interaction by 9 bp impaired rRNA processing. Formation of 18S rRNA was also inhibited by swapping the U3 5′- and 3′-hinge interactions with the ETS or by shifting the base pairing of the U3 3′-hinge to the sequence directly adjacent to ETS region E1. However, 18S rRNA production was partially restored by a compensatory shift that allowed the sequence adjacent to the U3 3′-hinge to pair with the eight bases directly adjacent to ETS region E1. The results suggest that the geometry of the U3 snoRNA interaction with the ETS is critical for rRNA processing.
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影响因子：
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作者：
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