Crucial role of the lipid part of lipopolysaccharide for conformational change of minor spike H protein of bacteriophage φX174
Crucial role of the lipid part of lipopolysaccharide for conformational change of minor spike H protein of bacteriophage φX174
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DOI：
10.1016/j.femsle.2005.08.014
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发表时间：
2005-10-15
影响因子：
2.1
通讯作者：
Nishikawa, S
中科院分区：
文献类型：
--
作者：
Inagaki, M;Wakashima, H;Nishikawa, S
通过比较脂多糖及其去酰化衍生物与H蛋白的相互作用，研究了脂多糖（LPS）的脂质部分对噬菌体phi X174小刺突H蛋白识别的作用。H蛋白的荧光和圆二色性（CD）光谱在与完整的LPS和部分去酰化衍生物结合后增加。完全去酰化后的H蛋白亲和度较低，几乎没有荧光和CD变化。这些结果表明LPS的脂质部分负责小刺突H蛋白的构象改变，该蛋白可以触发噬菌体DNA喷射以感染宿主细胞。(C) 2005年欧洲微生物学会联合会。Elsevier B.V.版权所有。
The contribution of the lipid part of lipopolysaccharide (LPS) to recognition by minor spike H protein of bacteriophage phi X174 was investigated by comparing the interactions of H protein with LPS and its deacylated derivatives. The fluorescence and circular dichroism (CD) spectra of H protein increased upon binding to intact LPS and a partially deacylated derivative. In contrast, completely deacylated derivatives showed lower affinities and almost no fluorescence or CD changes of H protein. These results demonstrate that the lipid part of LPS is responsible for the conformational change of minor spike H protein, which would function as a trigger for phage DNA ejection for infection of the host cell. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.