Comparison of removal performance of two surrogates for pathogenic waterborne viruses, bacteriophage Qβ and MS2, in a coagulation-ceramic microfiltration system
Comparison of removal performance of two surrogates for pathogenic waterborne viruses, bacteriophage Qβ and MS2, in a coagulation-ceramic microfiltration system
复制标题
DOI：
10.1016/j.memsci.2008.10.037
复制
发表时间：
2009-01-20
影响因子：
9.5
通讯作者：
Ohno, K.
中科院分区：
文献类型：
--
作者：
Shirasaki, N.;Matsushita, I.;Ohno, K.
在凝固陶瓷微滤过程中评估了两种致病性水传播病毒替代物（F 特异性 RNA 噬菌体 Q beta 和 MS2）的去除性能。添加了这些噬菌体的河水被用来研究它们行为的差异。分别通过噬菌斑形成装置和实时逆转录聚合酶链式反应方法测量感染性和总（感染性+灭活）噬菌体浓度。在每种凝固条件下，感染性 Q beta 和 MS2 的去除相似。在 1.08 mg-Al/L 凝固剂剂量和 5 分钟凝固时间下，两种噬菌体均实现了约 6 个对数的减少。即使当凝血剂剂量和凝血时间分别降低至 0.54 mg-Al/L 和 1.8 s 时，也至少出现 4 个对数级的减少。相反，总 Q beta 和 MS2 的去除明显不同。尽管 Q beta 和 MS2 的粒径和电泳迁移率相似，但总 MS2 的去除量比总 Q beta 的去除量大约大 2 个对数，这可能是由于与滤饼层的选择性相互作用。 4 小时过滤后，Q beta 和 MS2 保留在微滤室中的噬菌体总数相似，但感染性 Q beta 比感染性 MS2 少约 3 个对数，可能是由于对铝凝固剂杀病毒活性的敏感性不同。 (C) 2008 Elsevier B.V. 保留所有权利。
The removal performance of two surrogates for pathogenic waterborne viruses, F-specific RNA bacteriophages Q beta and MS2, was evaluated during the coagulation-ceramic microfiltration process. River water spiked with these bacteriophages was used to investigate differences in their behaviors. Infectious and total (infectious + inactivated) bacteriophage concentrations were measured by plaque forming unit and real-time reverse transcription-polymerase chain reaction methods, respectively. Removal of infectious Q beta and MS2 was similar under each coagulation condition. Approximately 6-log reduction was achieved for both bacteriophages at 1.08 mg-Al/L of coagulant dose and 5-min coagulation time. At least 4-log reduction occurred even when coagulant dose and coagulation time were reduced to 0.54 mg-Al/L and 1.8 s, respectively. In contrast, removal of total Q beta and MS2 differed markedly. Removal of total MS2 was approximately 2-log larger than that of total Q beta, possibly owing to selective interaction with the cake layer, although the particle diameters and electrophoretic mobilities of Q beta and MS2 were similar. The total number of bacteriophages retained in the microfiltration compartment after 4-h filtration was similar for Q beta and MS2, but there were approximately 3 log fewer infectious Q beta than infectious MS2, probably owing to the difference in sensitivity to the virucidal activity of aluminum coagulant. (C) 2008 Elsevier B.V. All rights reserved.