Possible involvement of antitumor immunity in the eradication of colon 26 induced by low-voltage electrochemotherapy with bleomycin
Possible involvement of antitumor immunity in the eradication of colon 26 induced by low-voltage electrochemotherapy with bleomycin
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DOI：
10.1007/s005950300006
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发表时间：
2003-01-01
期刊：
影响因子：
2.5
通讯作者：
Ochiai, T
中科院分区：
文献类型：
--
作者：
Miyazaki, S;Gunji, Y;Ochiai, T
目的。已有文献报道使用化疗药物和高压电脉冲进行电化疗的抗肿瘤效果。本研究旨在明确抗肿瘤免疫在化疗肿瘤结节消退过程中的作用，并评价低压电穿孔治疗的有效性。Balb/c小鼠和Balb/c nu/nu裸鼠分别皮下接种Colon 26细胞或metha细胞。采用博来霉素联合低压电穿孔（CUY21）治疗肿瘤结节。在肿瘤内（i.t）注射500马克博莱霉素，然后在50至150V/cm的电场范围内治疗，结肠26个肿瘤被根除，完全缓解率从80%到100%不等。小鼠拒绝再次接种结肠26细胞，但不拒绝甲基苯丙胺A细胞。在Balb/c nu/nu裸鼠中，在相同的治疗条件下，化疗后肿瘤未完全消退，而Balb/c裸鼠几乎完全治愈。我们的研究结果表明，t细胞依赖性、肿瘤特异性保护性免疫的产生可能参与了低压电疗中肿瘤结节消退的过程。
Purpose. The antitumor efficiency of electrochemotherapy using chemotherapeutic agents and high-voltage electric pulse has been reported. This study was done to define the precise nature of the involvement of antitumor immunity in the regression of tumor nodules in electrochemotherapy, and to evaluate the effectiveness of using low-voltage electroporation.Methods. Balb/c mice and Balb/c nu/nu nude mice were inoculated subcutaneously with Colon 26 cells or Meth A cells. Electrochernotherapy using bleomycin and low-voltage electroporation (CUY21) was performed as a treatment against tumor nodules.Results. Colon 26 tumors were eradicated in the mice given an intratumor (i.t.) injection of 500 mug bleomycin followed by treatment with electric fields ranging from 50 to 150V/cm, with complete response rates ranging from 80% to 100%. The mice rejected inoculations of rechallenged Colon 26 cells, but not Meth A cells. In the Balb/c nu/nu nude mice, complete regression of the tumor was not seen after electrochemotherapy under the same therapeutic conditions that resulted in almost complete cure in the Balb/c mice.Conclusion. Our results suggest that the generation of T-cell-dependent, tumor-specific protective immunity might be involved in the process of tumor nodule regression in low-voltage electrochemotherapy.