Formation of acellular cementum-like layers, with and without extrinsic fiber insertion, along inert bone surfaces of aging c-Src gene knockout mice.
Formation of acellular cementum-like layers, with and without extrinsic fiber insertion, along inert bone surfaces of aging c-Src gene knockout mice.
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沿着衰老的 c-Src 基因敲除小鼠的惰性骨表面形成无细胞牙骨质样层，有或没有外在纤维插入。
DOI：
10.1111/j.1600-0722.2006.00408.x
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发表时间：
2006
影响因子：
1.9
通讯作者：
Y. Takano
中科院分区：
文献类型：
--
作者：
O. Baba;Atsushi Miyata;T. Abe;S. Shibata;Y. Nakano;T. Terashima;Tsuyoshi Oda;A. Kudo;Y. Takano
为了研究c-src缺陷对骨骼和牙齿组织的长期影响，我们通过组织学和组织化学方法检测了c-src基因敲除（c-src KO）小鼠的下颌骨和长骨。许多多核破骨细胞分布在整个下颌骨在5周龄的c-src基因敲除小鼠，但到14周，他们几乎完全从牙槽骨消失，留下抗酒石酸酸性磷酸酶（TRAP）阳性层沿着骨表面。14周时，TRAP阳性骨表面沿着有骨桥蛋白阳性矿化组织沉积，类似于无细胞无纤维牙骨质（AAC）。该层逐渐增厚至21个月。沿长骨骨小梁沿着观察到相当的矿化层，作为增厚的骨水泥线。在颌骨富含骨膜蛋白的区域，而不是在长骨中，部分AAC样矿化层往往被替换和/或覆盖的脱细胞外源性纤维牙骨质（AEFC）样组织。这些数据表明，AAC样矿化组织的沉积是一种普遍现象，可能发生沿着惰性或缓慢重塑骨表面的条件下，其特征在于减少骨吸收活性，而AEFC样组织的诱导似乎与某些分子的表达，特别是丰富的牙周膜的微环境。
To investigate the long-term effects of c-src deficiency on skeletal and dental tissues, we examined the lower jaws and long bones of c-src gene knockout (c-src KO) mice by histological and histochemical methods. Numerous multinucleated osteoclasts were distributed throughout the mandible in 5-wk-old c-src KO mice, but by 14 wk they had almost completely disappeared from the alveolar bone, leaving tartrate-resistant acid phosphatase (TRAP)-positive layers along the bone surface. Deposition of osteopontin-positive mineralized tissue, reminiscent of acellular afibrillar cementum (AAC), was confirmed along the TRAP-positive bone surface at 14 wk. The layer progressively thickened up to 21 months. A comparable mineralized layer was noted along the trabeculae of long bones as thickened cement lines. In the periostin-rich areas of jaw bones, but not in the long bones, portions of AAC-like mineralized layers were often replaced with and/or covered by acellular extrinsic fiber cementum (AEFC)-like tissue. These data suggest that the deposition of AAC-like mineralized tissue is a general phenomenon that may occur along inert or slowly remodeling bone surfaces under conditions characterized by reduced bone-resorbing activity, whereas the induction of AEFC-like tissue seems to be associated with the expression of certain molecules that are particularly abundant in the microenvironment of the periodontal ligament.
DOI：
10.1073/pnas.90.10.4485
发表时间：
1993-05-15
影响因子：
11.1
作者：
LOWE, C;YONEDA, T;SORIANO, P
通讯作者：
SORIANO, P